AI Article Synopsis
- - The study aimed to understand how fibroblasts get activated in systemic sclerosis (SSc) by examining patients with different forms of the disease and comparing them to healthy individuals.
- - Researchers analyzed fibroblasts from skin biopsies, finding that those from SSc patients showed higher expression of GM-CSF receptors but did not grow more despite this stimulation; however, they did exhibit increased adhesion structures.
- - The findings suggest that the enhanced adhesion and differentiation of fibroblasts, linked to higher GM-CSF receptor expression, could play a significant role in the development of SSc.
Article Abstract
Objective: To elucidate events that initiate the involvement and stimulation of fibroblasts in systemic sclerosis (SSc).
Methods: We examined 15 patients with SSc diffuse form, 15 with CREST syndrome, and 5 healthy subjects. Cultured fibroblasts obtained from skin biopsies in SSc involved and non-involved areas and norrmal skin fibroblasts were cultured with different doses of granulocyte macrophage colony stimulating factor (GM-CSF) to study the effects of this factor on the expression of GM-CSF receptor (GM-CSFR) on fibroblast proliferation and cellular adhesion structures.
Results: Cultured fibroblasts obtained from biopsies of normal and SSc skin areas express GM-CSFR and such expression is increased in SSc fibroblasts. GM-CSF stimulation in vitro did not increase SSc fibroblast growth, in spite of a strongly increased expression of the GM-CSFR. The adhesion structures are always more abundant in SSc fibroblasts as compared to healthy cells and GM-CSF seems able to increase cell adhesion plaques.
Conclusion: We suggest that shift of fibroblasts toward a more adhesive differentiated pattern, due to or accompanied by an increased expression of GM-CSFR, may be an important event in the pathogenesis of SSc.
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