YidC was recently shown to play an important role in the assembly of inner membrane proteins (IMPs) both in conjunction with and separate from the Sec-translocon. Little is known about the biogenesis and structural and functional properties of YidC, itself a polytopic IMP. Here we analyze the targeting and membrane integration of YidC using in vivo and in vitro approaches. The combined data indicate that YidC is targeted by the signal recognition particle and inserts at the SecAYEG-YidC translocon early during biogenesis, unlike its mitochondrial homologue Oxa1p. In addition, YidC is shown to be relatively abundant compared with other components involved in IMP assembly and is predominantly localized at the poles of the cell.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1074/jbc.M200311200 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Role of Protein-Lipid Interactions in Priming the Bacterial Translocon.
Membranes (Basel)
November 2024
Department of Biochemistry, University of Illinois Urbana, Champaign, IL 61801, USA.
Protein-lipid interactions demonstrate important regulatory roles in the function of membrane proteins. Nevertheless, due to the semi-liquid nature and heterogeneity of biological membranes, and dissecting the details of such interactions at high resolutions continues to pose a major challenge to experimental biophysical techniques. Computational techniques such as molecular dynamics (MD) offer an alternative approach with both temporally and spatially high resolutions.
View Article and Find Full Text PDFFeatures of membrane protein sequence direct post-translational insertion.
Nat Commun
November 2024
Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel.
The proper folding of multispanning membrane proteins (MPs) hinges on the accurate insertion of their transmembrane helices (TMs) into the membrane. Predominantly, TMs are inserted during protein translation, via a conserved mechanism centered around the Sec translocon. Our study reveals that the C-terminal TMs (cTMs) of numerous MPs across various organisms bypass this cotranslational route, necessitating an alternative posttranslational insertion strategy.
View Article and Find Full Text PDFContact-mediated algicidal mechanism of ACE001 against the harmful alga Karenia mikimotoi.
iScience
November 2024
State Key Laboratory of Mariculture Breeding, Fisheries college of Jimei university, Xiamen, Fujian, P.R. China.
is a harmful algal bloom (HAB) species that poses a significant threat to marine ecosystems due to its hemolytic toxins. This study isolated (ACE001), which demonstrated contact-dependent algicidal effects against . Chemotaxis assays revealed ACE001's strong attraction to cell membranes, indicating the importance of chemotaxis.
View Article and Find Full Text PDFEffect of the Membrane Insertase YidC on the Capacity of to Secret Recombinant Proteins.
J Agric Food Chem
October 2024
School of Food and Biological Engineering, Anhui Fermented Food Engineering Research Center, Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, Hefei 230601, China.
is a crucial food-grade cell factory for secreting valuable peptides and proteins primarily via the Sec-dependent pathway. YidC, a membrane insertase, facilitates protein insertion into the lipid membrane for the translocation. However, the mechanistic details of how YidC affects protein secretion in remain elusive.
View Article and Find Full Text PDFCorrelation Analysis of Traditional Chinese Medicine Constitution and Metabolic Indexes in General Physical Examination People.
Endocr Metab Immune Disord Drug Targets
October 2024
Department of Prevention and Treatment of Disease Care Center, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, 130000, China.
Article Synopsis
- The study analyzed the relationship between Traditional Chinese Medicine (TCM) constitution types and metabolic indexes among 1,029 general patients from Changchun University Hospital between January 2021 and April 2023.
- It found that the balanced constitution (BC) was most common, while blood stasis constitution (BSC) was rare; various constitutional types showed significant differences in body mass index (BMI) and blood pressure levels.
- The study concluded that those with phlegm-dampness (PDC), yang-deficiency (YADC), and blood stasis (BSC) constitutions had more metabolic abnormalities compared to those with a balanced constitution (BC).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!