AI Article Synopsis
- The study aimed to evaluate serologic assays for S. aureus antibodies to differentiate various types of bacteremia and endocarditis.
- ELISAs were conducted on patients with different bacteremia types and controls to measure Ig G antibodies against specific S. aureus antigens.
- The whole-cell ELISA showed the highest sensitivity, but overall specificity was low; certain ELISA combinations could distinguish S. aureus from non-S. aureus endocarditis but failed to differentiate between uncomplicated and complicated S. aureus bacteremia.
Article Abstract
Objective: Serologic assays for Staphylococcus aureus antibodies were evaluated regarding their ability to differentiate between uncomplicated and complicated S. aureus bacteremia, between S. aureus and non-S. aureus bacteremia, and between S. aureus and non-S. aureus endocarditis.
Methods: Enzyme-linked immunosorbent assays (ELISAs) were performed to measure Ig G antibodies against seven S. aureus antigens (peptidoglycan, teichoic acid, S. aureus ultrasonicate, whole S. aureus cells, alpha-toxin, lipase and capsular polysaccharide) in 129 patients with S. aureus bacteremia (including 51 with endocarditis), 78 patients with non-S. aureus bacteremia (including 27 with endocarditis) and 100 febrile non-bacteremic controls.
Results: Whole-cell ELISA was the most sensitive assay. The specificity of all assays was low. Two different combinations of ELISAs for whole cells, teichoic acid,alpha-toxin, lipase and capsular polysaccharide did distinguish between S. aureus and non-S. aureus endocarditis, but not between uncomplicated and complicated S. aureus bacteremia.
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| http://dx.doi.org/10.1016/s0732-8893(01)00311-x | DOI Listing |
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