AI Article Synopsis
- The study compared kidney structures in different mouse strains, noting significant differences in vacuolar structures of the proximal convoluted tubules, particularly in male DBA/2 mice.
- The vacuoles in male DBA/2 mice exhibited unique staining properties and were identified as multilammelar giant dense bodies using electron microscopy.
- These structures were indicated to be giant lysosomes containing apolipoprotein B, showing heterogeneous reactions in their biochemical analysis.
Article Abstract
In the present study, we performed comparative histological observations of ICR, BALB/c, C57BL/6, C3H/HeN and DBA/2 mice kidneys. Sex and strain differences were observed in the appearance of vacuolar structures of the proximal convoluted tubules (toluidine blue-positive granules in osmium-postfixed epoxy-resin sections). These features were especially remarkable in male DBA/2 mice. The vacuolar structures in male DBA/2 mice showed heterogeneous staining with Sudan B in frozen sections and appeared under an electron microscope as multilammelar giant dense bodies. In addition, these dense bodies showed heterogeneous acid phosphatase reactions. Immunohistochemical analyses of these structures for apolipoprotein B showed strong positive reactions. These results suggested that vacuolar structures in the proximal convoluted tubules, which were remarkable in male DBA/2 mice, were giant lysosomes containing apolipoprotein B.
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| http://dx.doi.org/10.14670/HH-17.1 | DOI Listing |
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