SEL1L, the human orthologue of the Caenorhabditis elegans sel-1 gene, is differentially expressed in breast primary tumors and in normal breast tissues. Analysis of a series of human primary breast carcinomas, using a monoclonal antibody raised against a SEL1L recombinant protein, revealed down-modulation or absence of SEL1L expression in about two-thirds of the tumors as compared with normal breast epithelial cells. Overall survival analysis of breast carcinoma patients indicated a statistically significant correlation between SEL1L down-modulation and poor prognosis. MCF-7, human breast carcinoma cells, were transfected with a construct containing the entire SEL1L cDNA driven by an inducible promoter and showed a dramatic reduction in anchorage-dependent growth and colony formation in soft agar. Growth of the transfected cells in Matrigel, an extracellular matrix rich with laminin, restored colony-formation ability. These results point to the role for SEL1L in breast tumor growth and aggressiveness, possibly involving cell-matrix interactions.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Sex-specific transcriptomic profiling reveals key players in bone loss associated with Alzheimer's disease.
Geroscience
January 2025
Division of Endocrinology, Department of Medicine, Augusta University, Augusta, GA, USA.
Alzheimer's disease (AD), a progressive neurodegenerative disorder, is frequently associated with musculoskeletal complications, including sarcopenia and osteoporosis, which substantially impair patient quality of life. Despite these clinical observations, the molecular mechanisms linking AD to bone loss remain insufficiently explored. In this study, we examined the femoral bone microarchitecture and transcriptomic profiles of APP/PS1 transgenic mouse models of AD to elucidate the disease's impact on bone pathology and identify potential gene candidates associated with bone deterioration.
View Article and Find Full Text PDFObesity, insulin resistance, and a host of environmental and genetic factors can drive hyperglycemia, causing β-cells to compensate by increasing insulin production and secretion. In type 2 diabetes (T2D), β-cells under these conditions eventually fail. Rare β-cell diseases like congenital hyperinsulinism (HI) also cause inappropriate insulin secretion, and some HI patients develop diabetes.
View Article and Find Full Text PDFGenomic and transcriptomics analysis reveal putative secreted proteins expressed of during 18β-glycyrrhetinic acid treatment.
Front Vet Sci
November 2024
Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, China.
Article Synopsis
- A gram-negative pathogen can infect both animals and humans, causing significant economic damage to livestock.
- This study investigates the antibacterial properties of 18β-Glycyrrhetinic acid, identifying secreted proteins with eukaryotic-like domains that are regulated by this compound.
- Results indicate that 18β-Glycyrrhetinic acid affects bacterial energy metabolism and virulence factor expression, suggesting potential for alternative antibiotic therapies in managing infections.
Identification of new therapeutic targets related to endoplasmic reticulum stress and mitochondrial dysfunction to reduce the risk of rupture in degenerative ascending aortic aneurysm.
Clin Investig Arterioscler
October 2024
Facultad de Ciencias Básicas de la Salud, Universidad Rey Juan Carlos, Alcorcón, Madrid, España; CIBER de Enfermedades Cardiovasculares, CIBERCV, Instituto Carlos III (ISCIII), Madrid, España. Electronic address:
Background: Ascending Thoracic Aortic Aneurysm (ATAA) is a progressive dilation of the aorta that can be complicated by its dissection leading to death in 80-90% of the patients. When associated with aging and atherosclerosis, the outcome is worse and reconstructive surgery is the only effective therapy. Our objective was to characterize differential expressed genes (DEG) involved in endoplasmic reticulum (ER) and mitochondria dysfunction in patients with degenerative ATAA.
View Article and Find Full Text PDFTargeted metabolomics of muscle amino acid profles and hepatic transcriptomics analyses in grass carp () fed with broad beans.
Heliyon
October 2024
College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China.
While tissue amino acid compositions reflect that of the dietary protein source, and the liver orchestrates amino acid metabolism. In this study, we investigated the muscle amino acid profiles in ordinary and crisp grass carp. The 22 amino acids were measured, and seventeen showed significant concentration differences.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!