AI Article Synopsis
- A pair of vectors, pDV2 and pDV3, has been created for inserting DNA into Neurospora crassa at the his-3 locus.
- The insertion occurs between the his-3 gene and a recombination hot spot, enabling monitoring of meiotic recombination.
- The system allows for studying the effects of specific sequences on recombination and facilitating directed evolution of genes through diversification in living cells.
Article Abstract
We have constructed a pair of vectors, pDV2 and pDV3, that enable targeted insertion of exogenous DNA into Linkage Group I of Neurospora crassa at the his-3 locus. Transplaced sequences are inserted between his-3 and the cog(L) recombination hot spot and include his-3 mutations that allow meiotic recombination initiated by cog(L) to be monitored. Selection of correctly placed transforming DNA is based on complementation between different his-3 alleles borne by the plasmids and transformation hosts. The system allows investigation of the effect of any given sequence on recombination as well as diversification of sets of related sequences in vivo for directed evolution of genes.
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| http://dx.doi.org/10.1006/plas.2001.1549 | DOI Listing |
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