A strain devoid of the three hydrogenases characterized for Desulfovibrio fructosovorans was constructed using marker exchange mutagenesis. As expected, the H(2)-dependent methyl viologen reduction activity of the strain was null, but physiological studies showed no striking differences between the mutated and wild-type strains. The H(+)-D(2) exchange activity measured in the mutated strain indicates the presence of a fourth hydrogenase in D. fructosovorans.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC139505 | PMC |
| http://dx.doi.org/10.1128/JB.184.3.853-856.2002 | DOI Listing |
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