[Evaluation of 3 methods of DNA extraction from paraffin-embedded material for the amplification of genomic DNA using PCR].

Pesqui Odontol Bras

Disciplina de Patologia Bucal-Faculdade de Odontologia, USP.

Published: January 2002

There are several protocols reported in the literature for the extraction of genomic DNA from formalin-fixed paraffin-embedded samples. Genomic DNA is utilized in molecular analyses, including PCR. This study compares three different methods for the extraction of genomic DNA from formalin-fixed paraffin-embedded (inflammatory fibrous hyperplasia) and non-formalin-fixed (normal oral mucosa) samples: phenol with enzymatic digestion, and silica with and without enzymatic digestion. The amplification of DNA by means of the PCR technique was carried out with primers for the exon 7 of human keratin type 14. Amplicons were analyzed by means of electrophoresis in an 8% polyacrylamide gel with 5% glycerol, followed by silver-staining visualization. The phenol/enzymatic digestion and the silica/enzymatic digestion methods provided amplicons from both tissue samples. The method described is a potential aid in the establishment of the histopathologic diagnosis and in retrospective studies with archival paraffin-embedded samples.

Download full-text PDF

Source
http://dx.doi.org/10.1590/s1517-74912001000400008DOI Listing

Publication Analysis

Top Keywords

genomic dna
16
extraction genomic
8
dna formalin-fixed
8
formalin-fixed paraffin-embedded
8
paraffin-embedded samples
8
enzymatic digestion
8
dna
6
[evaluation methods
4
methods dna
4
dna extraction
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!