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Time course of Marburg and Ebola virus antigens expression in Vero cells was studied by indirect immunofluorescence test. The maximum accumulation of virus specific antigens in Vero cells infected with a high dose was observed after 48-54 h of incubation. It is essential for laboratory diagnosis that virus specific antigens can present as incorporations of different shape and size, starting from small hardly discernible granules (immediately after the virus adsorption) to large lumps, cords, accumulations, and diffuse fluorescence.
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