Combinations of 10 Cryptosporidium parvum oocysts, with various ratios of genotype I to genotype II, were isolated and subjected to PCR-restriction fragment length polymorphism analysis. Amplification of both genotypes in these samples ranged from 31 to 74% and yielded no information about the genotype proportions. In addition, since both genotypes were not always detected, amplification of a single genotype is not conclusive evidence that the sample contains only a single genotype.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC126566 | PMC |
| http://dx.doi.org/10.1128/AEM.68.1.427-429.2002 | DOI Listing |
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