Maximal responses to splanchnic nerve stimulation occurred in rats at the pulse width 0.5-1.0 Ohms regardless of the frequency. Peak constriction of arterioles and venules occurred at a 3-4-sec burst duration and 1-2-sec interval. Adrenergic blockade abolished the vasoconstriction in response to continuous nerve stimulation. However, the responses persisted in high-frequency burst stimulation, suggesting an involvement of non-adrenergic co-transmitter release. Thereupon, an efficient control of microvascular tone can be achieved by grouping the pulses into bursts or by an increase of the burst rate or duration.
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