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Rational Development of a Lipid Droplets and Hypochlorous Acid In-Sequence Responsive Fluorescent Probe for Accurate Imaging of Atherosclerotic Plaques.
Anal Chem
December 2024
Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China.
To answer the call for effective and timely intervention in cardiovascular diseases (CVDs), the development of fluorescent probes that can precisely identify atherosclerotic plaques, the root cause of various fatal CVDs, is highly desirable but remains a great challenge. Herein, by integrating bis(trifluoromethyl)benzyl and phenothiazine into the coumarin matrix, a robust fluorescent probe, NOR1, has been developed. NOR1 responds sequentially to lipid droplets (LDs) and HClO via fluorescence turn-on and ratiometric readouts, respectively, with a fast response rate (within 70 s for LDs and 80 s for HClO), excellent sensitivity (detection limit: 0.
View Article and Find Full Text PDFElectroencephalography and Anesthetic Depth in Children Under 2 Years of Age: A Prospective Observational Study.
Paediatr Anaesth
December 2024
Department of Anesthesiology and Pain Medicine, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea.
Background: Processed electroencephalogram (EEG) indices are widely used to monitor anesthetic depth. However, their reliability in children under 2 years of age remains questionable. During anesthesia maintenance in this age group, processed EEG indices frequently exhibit unexpectedly elevated values that exceed the intended target range.
View Article and Find Full Text PDFZinc(II) organic framework based bifunctional biomarker sensor for efficient detection of urinary 5-Hydroxyindoleacetic acid and serum 3-Nitrotyrosine.
Spectrochim Acta A Mol Biomol Spectrosc
December 2024
School of Chemistry and Chemical Engineering, North University of China, Taiyuan 030051, PR China. Electronic address:
Monitoring biomarker levels in body fluids is of great importance in clinical diagnosis. Herein, a robust 3D ZnMOF, {[Zn(BTPB)(bib)(μ-OH)]·2HO}, was fabricated based on the ligands of 1,4-bis(2,4,6-tricarboxylpyrid-5-yl)benzene (HBTPB) and 1,4-bis(imidazol-1-yl)benzene (bib). On the basis of its stable architecture and intrinsic luminescence, ZnMOF demonstrated remarkable potential as a bifunctional luminescent sensor for selective and sensitive detecting the biomarkers of 3-nitrotyrosine (3-NT) and 5-hydroxyindoleacetic acid (5-HIAA) in water and body fluids by employing distinct "turn-off" and "turn-on" responses.
View Article and Find Full Text PDFManagement and toxicological analysis of ocular hypertension after EyeCee ONE intraocular lens implantation: a case series.
BMC Ophthalmol
December 2024
Department 3, 15-20 National Vision Hospital, IHU FOReSIGHT, 28 rue de Charenton, Paris, 75012, France.
Background: The EyeCee ONE intraocular lens (Nidek, Gamagori, Japan) has been withdrawn from the market due to a high number of reports of severe ocular hypertension (OHT) following phacoemulsification with implantation of this intraocular lens (IOL). In this case series, we report the results of a toxicological analysis and the surgical management of five patients with severe OHT following the implantation of defective EyeCee ONE IOLs during cataract surgery.
Cases Presentation: Five patients developed early, severe OHT refractory to maximal medical therapy following uneventful phacoemulsification (PCE) cataract surgery with implantation of an EyeCee ONE IOL from a defective lot.
Transepithelial electrical resistance (TEER) measurement is a label free, rapid and real-time technique, which is commonly used to evaluate the integrity of cell barriers. TEER characterization is important for applications, such as tissue (brain, intestines, lungs) barrier modeling, drug screening, and cell growth monitoring. Traditional TEER methods usually only show the average impedance of the whole cell layer, and lack accuracy and the characterization of internal spatial differences within cell layer regions.
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