Background: Dental materials, in particular resins used in prosthetic, orthodontic, conservative and gnathological dentistry, are frequently in long-term contact with the tissues of the mouth, and the chronic exposure to these products of a significant proportion of the population means that the release of genotoxic substances by dental resins must be evaluated.
Methods: For this purpose, ten methyl metacrylate-based resins, both cold- and hot-polymerising and all of frequent use in clinical practice, were prepared following the indications in Standard UNI 9582-2 now included in Standard ISO 10993. Genotoxicity was evaluated through the Tradescantia/micronuclei test.
Results: By using the Tradescantia/micronuclei test, which detects aberrant chromosomes by detecting micronuclei in flower tetrads, nine resins were found not to the positive; one resin caused a significant increase in the frequency of micronuclei compared to negative controls.
Conclusions: The results of the Tradescantia/micronuclei test, together with the results of tests cytotoxicity and the Ames and Allium cepa tests performed in previous research, indicate a need for further analyses of these resins for dental use, through other in vivo and in vitro tests, in order to achieve certainty over the risk connected with their use in human populations.
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Assessment of the mutagenic potential of the water of an urban river by means of two Tradescantia-based test systems.
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Laboratory of General and Molecular Genetics, RI Biology, Faculty of Biology, Yerevan State University, 8, Charents Str., Yerevan, Armenia.
River pollution can be caused by anthropogenic or natural factors. When testing water quality for the presence of toxic substances, higher plants as bioindicators for the genotoxic effects of complex mixtures are effective and appropriate. Hence, in this work the Tradescantia (clone 02) stamen hair mutations (Trad-SHM) and Tradescantia micronuclei (Trad-MCN) were used to determine mutagenic and clastogenic potential of an urban river.
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Faculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Dourados, MS, Brazil.
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Dipartimento di Medicina Sperimentale ed Applicata, Università degli Studi di Brescia.
The aim of this research was to evaluate the potential genotoxic effects of three drinking water disinfectants by means of in vivo short-term mutagenicity tests using plants. The study was carried out in laboratory using distilled water disinfected with sodium hypochlorite (NaClO), chlorine dioxide (ClO2) and peracetic acid (PAA) at different concentrations both in neutral and acid conditions. Untreated distilled water was used as a negative control.
View Article and Find Full Text PDF[Genotoxic and ecotoxic effects of urban waste water disinfected with sodium hypochlorite or peracetic acid].
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Genotoxic and ecotoxicologic effects of urban wastewater disinfected with sodium hypochlorite or peracetic acid were analyzed. The formation of genotoxic species was studied by determining clastogenic and mutagenic activity of aqueous samples and their extracts with in vivo and in vitro tests, respectively. In particular, we have applied citogenetic tests to Allium cepa roots and Tradescantia inflorescence (Allium cepa test and Tradescantia/micronuclei test) and reversion test to Salmonella typhimurium according to the microsuspension procedure (Kado test).
View Article and Find Full Text PDFEvaluation of the migration of mutagens/carcinogens from PET bottles into mineral water by Tradescantia/micronuclei test, Comet assay on leukocytes and GC/MS.
Sci Total Environ
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Department of Life Science, II University of Naples, Naples, Italy.
This study monitored the release of mutagenic/carcinogenic compounds into mineral water (natural and carbonated) from polyethylene terephthalate (PET) bottles, using a plant mutagenicity test which reveals micronuclei formation in Tradescantia pollen cells (Trad/MCN test), a DNA damage assay (Comet assay) on human leukocytes and gas chromatography/mass spectrometry (GC/MS) for the characterisation of migrants. The water samples were collected at a bottling plant and stored in PET bottles for a period ranging from 1 to 12 months. Every month some samples were randomly collected and lyophilised, the residual powders were extracted with organic solvents and then analysed by GC/MS and tested for DNA damage in human leukocytes, or reconstituted with distilled water to obtain concentrates for the exposure of Tradescantia inflorescences.
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