An insulin-receptor substrate of 53-kDa protein (IRSp53) is an adapter protein, which interacts with the Rho-family of GTPases and mediates neurite outgrowth. It also binds to DRPLA protein, a product of the gene responsible for a polyglutamine disease, dentatorubral-pallidoluysian atrophy (DRPLA). Isoforms of human IRSp53 have been reported, each with a unique amino acid sequence at the C-terminal end. Here we report the distinctive tissue distribution and phosphorylation of three isoforms (L, S, and T-forms). Western blotting analyses with isoform-specific antibodies demonstrated that the L and S-forms were expressed in the brain, whereas the T-form was not present in any tissues examined, but was found in a cancer cell line. The L and S-forms were phosphorylated upon stimulation with insulin, and the T-form with IGF-I. Since phospho-acceptor sites were localized to the common portion, the difference in phosphorylation seems to be due to the unique C-terminal sequence.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1006/bbrc.2001.6102 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring the Unique Properties and Superior Schwann Cell Guiding Abilities of Spider Egg Sac Silk.
ACS Appl Bio Mater
January 2025
Institute of Physics and Materials Science, Department of Natural Sciences and Sustainable Ressources, BOKU University, Peter Jordan-Straß 82, 1190 Vienna, Austria.
Spider silk (SPSI) is a promising candidate for use as a filler material in nerve guidance conduits (NGCs), facilitating peripheral nerve regeneration by providing a scaffold for Schwann cells (SCs) and axonal growth. However, the specific properties of SPSI that contribute to its regenerative success remain unclear. In this study, the egg sac silk of is investigated, which contains two distinct fiber types: tubuliform (TU) and major ampullate (MA) silk.
View Article and Find Full Text PDFCollagen turnover during cervical remodeling involves both intracellular and extracellular collagen degradation pathways.
Biol Reprod
January 2025
Department of Ob/Gyn and Cecil H. and Ida Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical Center, Dallas, Texas, USA.
Reproductive success requires accurately timed remodeling of the cervix to orchestrate the maintenance of pregnancy, the process of labor, and birth. Prior work in mice established that a combination of continuous turnover of fibrillar collagen and reduced formation of collagen cross-links allows for the gradual increase in tissue compliance and delivery of the fetus during labor. However, the mechanism for continuous collagen degradation to ensure turnover during cervical remodeling is still unknown.
View Article and Find Full Text PDFChirality Interplay of Peptide and Saccharide on Glycopeptide Self-Assembly.
Nano Lett
January 2025
Zhejiang Engineering Research Center for Tissue Repair Materials and Wenzhou Key Laboratory of Biomaterials and Engineering and Wenzhou Key Laboratory of Biophysics, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, China.
Saccharides and peptides with markedly disparate stereochemical features serve as pivotal chiral molecular partners in living systems. The importance of glycosylation in influencing glycopeptide self-assembly has been recognized. However, how different chiral combinations of saccharides and peptides influence the macroscopic hydrogel mechanics, fiber nanomechanics, asymmetric molecular packing, and thermodynamic changes during glycopeptide self-assembly remains unknown.
View Article and Find Full Text PDFGenome-Wide Analysis of the Gene Family in .
Plant Direct
January 2025
Provincial Key Laboratory of Conservation and Utilization of Traditional Chinese Medicine Resources, Institute of Chinese Herbal Medicines Henan Academy of Agricultural Sciences Zhengzhou China.
The superfamily represents a class of transcription factors involved in plant growth, development, and stress responses. ., also known as safflower, is an important plant whose flowers contain carthamin, an expensive aromatic pigment with various medicinal and flavoring properties.
View Article and Find Full Text PDFComparative analysis of intraoperative thermal and optical imaging for identification of the human primary sensory cortex.
J Biomed Opt
January 2025
TU Dresden, Carl Gustav Carus Faculty of Medicine, Anesthesiology and Intensive Care Medicine, Clinical Sensing and Monitoring, Dresden, Germany.
Significance: The precise identification and preservation of functional brain areas during neurosurgery are crucial for optimizing surgical outcomes and minimizing postoperative deficits. Intraoperative imaging plays a vital role in this context, offering insights that guide surgeons in protecting critical cortical regions.
Aim: We aim to evaluate and compare the efficacy of intraoperative thermal imaging (ITI) and intraoperative optical imaging (IOI) in detecting the primary somatosensory cortex, providing a detailed assessment of their potential integration into surgical practice.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!