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Characterization and genome analysis of lytic Vibrio phage VPK8 with potential in lysing Vibrio parahaemolyticus isolates from clinical and seafood sources.
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Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Background: Vibrio parahaemolyticus is a marine bacterium causing seafood-associated gastrointestinal illness in humans and acute hepatopancreatic necrosis disease (AHPND) in shrimp. Bacteriophages have emerged as promising biocontrol agents against V. parahaemolyticus.
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Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.
Azurin a potent anticancer and antimicrobial agent isolated from a novel Pseudomonas aeruginosa strain.
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Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, Egypt.
Azurin, a bacterial blue-copper protein, has garnered significant attention as a potential anticancer drug in recent years. Among twenty Pseudomonas aeruginosa isolates, we identified one isolate that demonstrated potent and remarkable azurin synthesis using the VITEK 2 system and 16S rRNA sequencing. The presence of the azurin gene was confirmed in the genomic DNA using specific oligonucleotide primers, and azurin expression was also detected in the synthesized cDNA, which revealed that the azurin expression is active.
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View Article and Find Full Text PDFExtraction, Isolation, and Structural Elucidation of Acylated Triterpene Saponins from Asteraceae Family.
Methods Mol Biol
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College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, Republic of Korea.
Saponins represent specialized (secondary) metabolites primarily sourced from plants, typically characterized by an aglycone component of triterpenoids or steroids, often referred to as sapogenin, coupled with sugar moieties. Their structural intricacy and diversity, along with their manifold pharmacological properties, have garnered significant interest among researchers. Notwithstanding this interest, the study of saponins has been encumbered by challenges in their isolation, purification, and structural characterization.
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