Degradation of cellulose by nitrogen-fixing strain of Bacillus polymyxa.

Acta Microbiol Pol

Department of Agricultural Microbiology, Warsaw Agricultural University, 02-528 Warsaw, 26/30 Rakowiecka.

Published: April 2002

During an intensive screening programme, several strains of cellulolytic bacteria were isolated. One nitrogenase-positive strain able to degrade filter paper, Avicel cellulose, carboxymethyl cellulose and cellobiose was selected for further study. On the basis of biochemical characteristics and Mol % of G+C content, the selected strain was identified as Bacillus polymyxa. The highest production of the enzymes degrading filter paper (FP-ase) and carboxymethyl cellulose (CMCase) by B. polymyxa was observed in Park's medium suplemented with Avicel cellulose. The investigated strain of bacteria produced cellulosome-like structures as was shown by transmission electron microscopy.

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