AI Article Synopsis
- The study describes the cloning and initial characterization of the Pit-2 gene promoter, which is responsible for a type III sodium-dependent phosphate cotransporter in mice.
- Five different promoter/luciferase reporter gene constructs were tested, ranging in size from -1816 to -225 base pairs, demonstrating significant luciferase activity (6-14 times higher than the background level) when these constructs were introduced into human colon cancer cells (Caco-2) and opossum kidney cells (OKP).
- The results suggest that the cloned promoter is functionally active and can drive gene expression, which may be important for understanding phosphate transport mechanisms in different cell types.
Article Abstract
We report the novel cloning and preliminary characterization of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter. Five promoter/luciferase reporter gene constructs, -1816/+61, -1620/+61, -1223/+61, -600/+61 and -225/+61, showed significant luciferase activity (6-14-fold over background) when transfected into human colon carcinoma (Caco-2) and opossum kidney (OKP) cells.
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| http://dx.doi.org/10.1016/s0167-4781(01)00297-4 | DOI Listing |
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