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Characterization of the depolymerizing activity of commercial lipases and detection of lipase-like activities in animal organ extracts using poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thin film.
AMB Express
December 2016
School of Biological Sciences, Universiti Sains Malaysia, 11800, USM, Penang, Malaysia.
Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is one of the polyhydroxyalkanoate (PHA) copolymers which can be degraded by lipases. In this study, the depolymerizing activity of different known commercial lipases was investigated via microassay using P(3HB-co-92 mol % 4HB) thin film as substrate. Non-enzymatic hydrolysis occurred under conditions in which buffers with pH 12 and 13 were added or temperature of 50 °C and above.
View Article and Find Full Text PDFDensitometry based microassay for the determination of lipase depolymerizing activity on polyhydroxyalkanoate.
AMB Express
May 2013
Ecobiomaterial Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Penang, 11800, Malaysia.
A novel method for the assay of polyhydroxyalkanoate (PHA)-degrading ability of triacylglycerol lipases was developed. By applying the natural affinity of lipases towards hydrophobic interfaces, a sensitive and rapid densitometry analysis for the evaluation of hydrolytic activity of lipase droplets towards PHA-coated surface was successfully carried out. We found that 12 out of 14 tested lipases which are of fungal, bacterial and animal origin were able to hydrolyze P(3HB-co-92 mol% 4HB) thin film.
View Article and Find Full Text PDFInhibition of Propionibacterium acnes lipase by extracts of Indian medicinal plants.
Int J Cosmet Sci
June 2012
Department of Biochemistry, The Institute of Science, Madam Cama Road, Mumbai 400032 India.
Lipases play an important role in pathogenesis of acne by hydrolysing sebum triglycerides and releasing irritating free fatty acids in the pilosebaceous follicles. Lipase is a strong chemotactic and proinflammatory antigen. Therefore, lipase has generated a high interest as a pharmacological target for antiacne drugs.
View Article and Find Full Text PDFBiological Activities of a Mixture of Biosurfactant from Bacillus subtilis and Alkaline Lipase from Fusarium oxysporum.
Braz J Microbiol
January 2011
Departamento de Ciência de Alimentos, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas , Campinas, SP , Brasil ;
In this study, we investigate the antimicrobial effects of a mixture of a biosurfactant from Bacillus subtilis and an alkaline lipase from Fusarium oxysporum (AL/BS mix) on several types of microorganisms, as well as their abilities to remove Listeria innocua ATCC 33093 biofilm from stainless steel coupons. The AL/BS mix had a surface tension of around 30 mN.m(-1), indicating that the presence of alkaline lipase did not interfere in the surface activity properties of the tensoactive component.
View Article and Find Full Text PDFMicroassays for a set of enzymes in individual small marine copepods.
Comp Biochem Physiol A Mol Integr Physiol
November 2006
Biologische Anstalt Helgoland, Foundation Alfred Wegener Institute for Polar and Marine Research, P. O. Box 180, D-27483 Helgoland, Germany.
Fluorogenic assays for a set of five hydrolytic enzymes involved in digestion and food utilization (alanine and arginine aminopeptidase, lipase/esterase, chitobiase, and beta-glucosidase) were optimized to measure activities of these enzymes in the same extracts of individual small North Sea copepods. The enzyme activities of Acartia clausi, Centropages typicus, Corycaeus anglicus, Paracalanus parvus, and Temora longicornis showed distinct species specific activity patterns, but also high intra-specific variability. Protein, lipids, carbon and nitrogen (C, N) were determined with micro-scale assays in individual copepods or in batches of 10 to 50 animals.
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