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Rhodamine-functionalized carbon dots with pH-regulated FRET efficiency for ratiometric fluorescence sensing and imaging of extremely alkaline pH.
Mikrochim Acta
January 2025
School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, 421001, China.
A ratiometric fluorescent nanoprobe (CDs-Rho), synthesized through the simple covalent amide linkage between carbon dots (CDs) and pH-sensitive rhodamine dye (Rho), was designed for the precise sensing and imaging of extremely alkaline environments. The sensing mechanism involves the opposite pH-dependent fluorescence changes in CDs and Rho, respectively, coupled with pH-regulated FRET efficiency from CDs to Rho. The nanoprobe features a wide pH response window from pH 7.
View Article and Find Full Text PDFA Y178C rhodopsin mutation causes aggregation and comparatively severe retinal degeneration.
Cell Death Discov
January 2025
Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, OH, USA.
Rhodopsin is the light-activated G protein-coupled receptor that initiates vision in photoreceptor cells of the retina. Numerous mutations in rhodopsin promote receptor misfolding and aggregation, causing autosomal dominant retinitis pigmentosa, a progressive retinal degenerative disease. The mechanism by which these mutations cause photoreceptor cell death, and the role aggregation plays in this process is still unclear.
View Article and Find Full Text PDFCellulose-enhanced MoS bifunctional hydrogel for efficient methylene blue degradation, human body sensing, and recyclability.
Int J Biol Macromol
January 2025
School of Chemical Engineering, Changchun University of Technology, Changchun 130012, China.
In this study, the dispersion behavior of MoS₂ in ionic liquids (ILs) with varying alkyl chain lengths was the primary focus of investigation, followed by the design of a novel PAM/SMA/CMC/PDA@MoS hydrogel. By optimizing the concentrations of CMC and PDA@MoS, a bifunctional hydrogel with both sensing and catalytic functions was successfully developed. Mechanical tests revealed that the PAM/SMA/CMC/0.
View Article and Find Full Text PDFA first morphological and electrophysiological characterization of Fañanas cells of the mouse cerebellum.
J Physiol
January 2025
Université Paris Cité, CNRS, Saints-Pères Paris Institute for the Neurosciences, Paris, France.
Fañanas cells (FCs) are cerebellar glia of unknown function. First described more than a century ago, they have been almost absent from the scientific literature ever since. Here, we combined whole-cell, patch clamp recordings, near-UV laser photolysis, dye-loading and confocal imaging for a first characterization of FCs in terms of their morphology, electrophysiology and glutamate-evoked currents.
View Article and Find Full Text PDFUse of Indocyanine Green (ICG) to Assess Myocardial Perfusion and Territorial Distribution of Vein Grafts Implanted on Coronary Arteries in an Porcine Model. A Potential Adjunct to Assist Revascularization Strategies and Training in Coronary Artery Bypass Grafting.
Rev Cardiovasc Med
January 2025
Cardiac Surgery, University of Cincinnati Medical Center, Cincinnati, OH 45202, USA.
Background: The fluorescent dye indocyanine green (ICG) has been used to identify anatomical structures intraoperatively in coronary artery bypass grafting (CABG). This study aimed to evaluate the feasibility of using ICG to assess graft patency and territorial distribution of myocardial reperfusion during CABG.
Methods: Porcine arrested hearts (n = 18) were used to evaluate territorial distribution of native coronary arteries and of a coronary bypass constructed with porcine saphenous vein graft (SVG) using ICG.
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