CTLs recognize peptide epitopes which are proteolytically generated by the proteasome and presented on MHC class I molecules. According to the defective ribosomal product (DRiP) hypothesis, epitopes originate from newly synthesized polypeptides which are degraded shortly after their translation. The DRiP hypothesis would explain how epitopes can be generated from long-lived proteins. We examined whether neosynthesis is required for presentation of the immunodominant epitope NP118 of the lymphocytic choriomeningitis virus nucleoprotein, which has a half-life of >3 days. Two days after nucleoprotein biosynthesis was terminated in a tetracycline-regulated transfectant, the presentation of the NP118 epitope ceased. This indicates that NP118 epitopes are generated from newly synthesized nucleoproteins rather than from the long-lived pool of nucleoproteins in the cell. Therefore, the lymphocytic choriomeningitis virus nucleoprotein is the first substrate for which a major prediction of the DRiP hypothesis, namely the requirement for neosynthesis, is shown to hold true.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.4049/jimmunol.167.9.4801 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Making dew in the Atacama Desert: a paradigmatic case of plant water uptake water from an unsaturated atmosphere fails a test.
Ann Bot
December 2024
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.
Background And Aims: Nolana mollis is a dominant plant species in the hyperarid Atacama Desert. A previous hypothesis states that N. mollis owes its success to the condensation of atmospheric water from undersaturated air onto its leaf surfaces by exuded salts, and absorption of this water by its leaves, or by shallow roots following drip onto the soil surface; living roots of N.
View Article and Find Full Text PDFEosinophilic esophagitis and inhalant antigens: Pointing out the roles of allergic rhinitis, immunotherapy and biologic treatment.
World Allergy Organ J
October 2024
Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, Italy.
Article Synopsis
- - Eosinophilic esophagitis (EoE) and allergic rhinitis (AR) are linked through a common type 2 inflammation response, with AR frequently occurring in EoE patients and both being influenced by aeroantigens and seasonal factors.
- - Current research struggles to establish clear conclusions regarding the relationship between EoE and AR due to study designs that may not adequately account for various external factors, making pollen counts a potentially more reliable indicator of EoE exacerbations.
- - Allergen immunotherapy's effects on EoE symptoms are still uncertain, with sublingual immunotherapy typically causing exacerbations while subcutaneous immunotherapy may aid in remission; monoclonal antibodies may offer future treatment possibilities
Effectiveness of three nitrification inhibitors on mitigating trace gas emissions from different soil textures under surface and subsurface drip irrigation.
J Environ Manage
May 2024
Institute of Plant Nutrition and Soil Science, Kiel University, Hermann-Rodewald-Strasse 2, 24118, Kiel, Germany. Electronic address:
Nitrification inhibitors (NIs) and drip irrigation are recommended to mitigate trace gas emissions from agricultural soils. However, studies comparing the effect of different NIs on the release of trace gases from soils with contrasting textures under subsurface (SBD) and surface (SD) drip irrigation are lacking. Therefore, this study aimed to assess the effectiveness of three NIs in mitigating nitrous oxide (NO), carbon dioxide (CO), and methane (CH) emissions from two soils with different textures under SBD, with pipe buried in 10 cm depth, and SD.
View Article and Find Full Text PDFEnricherator: A Bayesian Method for Inferring Regularized Genome-wide Enrichments from Sequencing Count Data.
J Mol Biol
September 2024
Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA; Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address:
A pervasive question in biological research studying gene regulation, chromatin structure, or genomics is where, and to what extent, does a signal of interest arise genome-wide? This question is addressed using a variety of methods relying on high-throughput sequencing data as their final output, including ChIP-seq for protein-DNA interactions, GapR-seq for measuring supercoiling, and HBD-seq or DRIP-seq for R-loop positioning. Current computational methods to calculate genome-wide enrichment of the signal of interest usually do not properly handle the count-based nature of sequencing data, they often do not make use of the local correlation structure of sequencing data, and they do not apply any regularization of enrichment estimates. This can result in unrealistic estimates of the true underlying biological enrichment of interest, unrealistically low estimates of confidence in point estimates of enrichment (or no estimates of confidence at all), unrealistic gyrations in enrichment estimates at very close (<10 bp) genomic loci due to noise inherent in sequencing data, and in a multiple-hypothesis testing problem during interpretation of genome-wide enrichment estimates.
View Article and Find Full Text PDFReplacement of Vitamin E by an Extract from an Olive Oil By-Product, Rich in Hydroxytyrosol, in Broiler Diets: Effects on Growth Performance and Breast Meat Quality.
Antioxidants (Basel)
October 2023
Departamento Producción Agraria, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, Avda. Puerta de Hierro 2, 28040 Madrid, Spain.
The hypothesis of this experiment was that a liquid rich in hydroxytyrosol (HT) obtained from "alperujo", an olive oil by-product, could replace part of the added vitamin E (VE) as an antioxidant in poultry diets. There were five diets that differed exclusively in the substitution of supplemental VE (0 to 40 mg/kg, with differences of 10 mg/kg) by HT (30 to 0 mg/kg, with differences of 7.5 mg/kg).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!