A modification is described for the method of ultramicroelectrophoretic fractioning of proteins in polyacrylamide gel in capillaries 400--100 microns in diameter. The original construction of the assembly of apparatuses is offered which can be assembled from details made in the USSR. The operation of dosed filling of capillaries is significantly facilitated. The optimal quantity of protein in one sample reaches 1.5--0.1 mkg (depending on the capillary diameter). The reproducability and sharpness of protein fractions by the offered method are not worse compared with the generally accepted method of macro-disc electrophoresis.
Download full-text PDF |
Source |
---|
Biull Eksp Biol Med
May 1977
The ultramicroelectrophoretic method was applied to the study of the lactic dehydrogenase (LDH) spectrum alterations during the developmental phases of mice: ovulated ova--zygote--blastocyte--embryo--oocyte--ovulated ova. Only H-subunits were found in the embryo cells before the 5th day of development. After this M-subunits appeared indicating derepression of LDH-A locus.
View Article and Find Full Text PDFBiull Eksp Biol Med
September 1975
The tentative optimal values of the protein sample quantities were determined for the ultramicroelectrophoretic fractionation in the polyacrylamide gels in the capillaries of different diameters which provided distinct and highly reproducible electrophoregramms. A simple calculation (by means of table and formula) of the volumes of protein solutions required for different concentrations and different diameters of capillaries is suggested.
View Article and Find Full Text PDFA modification is described for the method of ultramicroelectrophoretic fractioning of proteins in polyacrylamide gel in capillaries 400--100 microns in diameter. The original construction of the assembly of apparatuses is offered which can be assembled from details made in the USSR. The operation of dosed filling of capillaries is significantly facilitated.
View Article and Find Full Text PDFMethods for staining of ultramicroenzymelectrophoregrams are described. Sharp and well reproducible bands of isozymes of LDH, MDH, G-6-PD, AcP, AlP, alpha-NAE were obtained from homogenates of cultivated human fibroblasts, of rat's liver, kidney and brain, of human embryonic liver, and in blood serum of man and frog. An extract from the rat brain containing only 3.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!