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A dual expression plasmid with Microcin B17 compatible with both prokaryotic and mammalian systems.
MethodsX
June 2025
Department of Pharmacology, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
Proteic plasmid addiction systems, such as the control of cell death (Ccd), have been used for efficient plasmid DNA recombination. The CcdB toxin, which has a relatively long sequence of 309 bp, has been the predominant choice for this purpose. However, the need for shorter peptide toxins has emerged.
View Article and Find Full Text PDFHigh-risk clones harboring β-lactamases: 2024 update.
Heliyon
January 2025
Unidad de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Carbapenem-resistant is defined by the World Health Organization as a "high priority" in developing new antimicrobials. Indeed, the emergence and spread of multidrug-resistant (MDR) or extensively drug-resistant (XDR) bacteria increase the morbidity and mortality risk of infected patients. Genomic variants of that display phenotypes of MDR/XDR have been defined as high-risk global clones.
View Article and Find Full Text PDFInter- and intra-varietal clonal differences influence the aroma compound profiles of wines analyzed by GC-MS and GC-IMS.
Food Chem X
January 2025
College of Enology, Northwest A & F University, No. 22 Xinong Road, Yangling 712100, China.
To investigate the impact of genetic factors on wine aroma, wines made from 22 clones of five grape varieties ( L.) were used to analyze the volatile compounds by headspace solid phase microextraction gas chromatography mass spectrometer (HS-SPME-GC-MS) and headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS). Results showed that 52 and 49 aroma compounds were identified from 22 clones of wines by two technologies, respectively.
View Article and Find Full Text PDFColorectal carcinogenesis in the Lynch syndromes and familial adenomatous polyposis: trigger events and downstream consequences.
Hered Cancer Clin Pract
January 2025
Division of Cancer and Genetics, Cardiff University School of Medicine, Heath Park, Cardiff, CF14 4XN, UK.
Carcinogenesis encompasses processes that lead to increased mutation rates, enhanced cellular division (tumour growth), and invasive growth. Colorectal cancer (CRC) carcinogenesis in carriers of pathogenic APC (path_APC) and pathogenic mismatch repair gene (path_MMR) variants is initiated by a second hit affecting the corresponding wild-type allele. In path_APC carriers, second hits result in the development of multiple adenomas, with CRC typically emerging after an additional 20 years.
View Article and Find Full Text PDFDiversity of hemipterans in Eucalyptus spp. plantations in southeastern pará, Eastern Amazon, Brazil.
Neotrop Entomol
January 2025
Institute of Agricultural Sciences, Federal Rural University of the Amazon - UFRA, Belém, PA, Brazil.
Brazil has 10.2 million hectares of reforestation, which account for 81% of the timber produced in the country. The order Hemiptera contains the main phytophagous species.
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