Studies of cell lines obtained as a result of in vitro transformation by bovine adenovirus type 3 at low multiplicities of infection: BHK-21/13-780 and BHK-21/13-782 (8 X 10(-5) TCD50/cell) and hamster embryo tissue HET-1 and HET-2 (2 X 10(-3) TCD50/cell) showed them to possess some oncologic characteristics typical of spontaneous neoplasia such as low degree of differentiation, high rate of cell growth, reduced adhesiveness, high tumorigenicity. In hamster embryo tissue cells (HET) transformed in vitro virus-specific transplantation antigen and S-antigen were found. In transformed cells of the initial BHK-21/13S culture possessing the capacity of transforming spontaneously and under the effect of oncogenic viruses no transplantation antigen coded for by bovine adenovirus type 3 were detected. It is suggested that the above-described transformed cell lines be used as experimental models for investigation of the processes of transformation and tumour progression.
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