Objective: To investigate the chemical constituents of Tetrastigma hemsleyanum.

Method: Compounds were separated by rechromatography on silica gel from the chloroform solubles of its ethanol extracts, and the structures were determined by spectral analysis and chemical evidence.

Result: Three compounds were isolated and elucidated as 6'-O-benzoyldaucosterol, daucosterol and beta-sitosterol.

Conclusion: All the three compounds were isolated from this plant for the first time, of which 6'-O-benzoyldaucosterol is a new natural product.

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