Enzyme therapy for the prevention and treatment of organophosphate poisoning depends on the availability of large amounts of cholinesterases. Transgenic plants are being evaluated for their efficiency and cost-effectiveness as a system for the bioproduction of therapeutically valuable proteins. Here we report production of a recombinant isoform of human acetylcholinesterase in transgenic tomato plants. Active and stable acetylcholinesterase, which retains the kinetic characteristics of the human enzyme, accumulated in tomato plants. High levels of specific activity were registered in leaves (up to 25 nmol min(-1) mg protein(-1)) and fruits (up to 250 nmol min(-1) mg protein(-1)).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/bit.10012 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Non-targeted metabolomics reveals fatty acid and associated pathways driving resistance to whitefly and tomato leafminer in wild tomato accessions.
Sci Rep
January 2025
World Vegetable Center, 60 Yi-Min Liao, Shanhua, Tainan, 74151, Taiwan.
Wild tomato species exhibit natural insect resistance, yet the specific secondary metabolites and underlying mechanisms governing the resistance remain unclear. Moreover, defense expression dynamically adapts to insect herbivory, causing significant metabolic changes and species-specific secondary metabolite accumulation. The present study aims to identify the resistance-related metabolites in wild tomato accessions that influence the defense mechanism against whitefly (Bemisia tabaci Asia II 7) and leafminer (Phthorimaea absoluta).
View Article and Find Full Text PDFTomato B-cell lymphoma2 (Bcl2)-associated athanogene 5 (SlBAG5) contributes negatively to immunity against necrotrophic fungus Botrytis cinerea through interacting with SlBAP1 and modulating catalase activity.
Int J Biol Macromol
January 2025
Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, PR China; Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, PR China. Electronic address:
The evolutionarily conserved and multifunctional B-cell lymphoma2 (Bcl2)-associated athanogene proteins (BAGs), serving as co-chaperone regulators, play a pivotal role in orchestrating plant stress responses. In this study, the possible involvement of tomato SlBAG genes in resistance to Botrytis cinerea was examined. The SlBAG genes respond with different expression change patterns to B.
View Article and Find Full Text PDFQuantification of Escherichia coli transfer from plastic mulch to field-grown tomatoes and bell peppers.
J Food Prot
January 2025
Department of Horticulture, University of Georgia, 1111 Miller Plant Sciences, Athens, GA, 30602, USA.
In the United States, the Food and Drug Administration (USFDA) prohibits distribution of fruit that is dropped from the plant and contacts the ground during harvest. This includes fruit which contacts the ground while attached to the plant, called "drooping" fruit. In the Southeastern US, tomato and pepper are trellised and grown on plastic mulch.
View Article and Find Full Text PDFHealth risk assessment of potential heavy metals bioaccumulation in specialty crops grown in farm soils amended with dredged material.
J Environ Manage
January 2025
The Ohio State University, Department of Horticulture and Crop Science, 202 Kottman Hall, 2021 Coffey Rd, Columbus, OH, 43210, USA.
This research examined the potential human health risk associated with heavy metal bioaccumulation in specialty crops (lettuce, tomato, carrot) grown in soils amended with dredged material (DM) from the Toledo Harbor in Ohio, USA. The specialty crops were chosen to expand the scope of the study of dredged material, especially in Northwest Ohio. Previous studies have been focused on corn and soybeans, which are the major crops planted in the area.
View Article and Find Full Text PDFAutoPVPrimer: A comprehensive AI-Enhanced pipeline for efficient plant virus primer design and assessment.
PLoS One
January 2025
Department of Surgical and Medical Sciences, Magna Graecia University of Catanzaro, Catanzaro, Italy.
Plant viruses pose a significant threat to global agriculture and require efficient tools for their timely detection. We present AutoPVPrimer, an innovative pipeline that integrates artificial intelligence (AI) and machine learning to accelerate the development of plant virus primers. The pipeline uses Biopython to automatically retrieve different genomic sequences from the NCBI database to increase the robustness of the subsequent primer design.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!