The cortical actinin contents was found to be higher in the inner medulla of the rat kidney. Tropomyosin was distributed differently: it is lower in the cortex than in outer or inner medulla. The processes controlled by these proteins seem to be important for the vasopressin renal effects, and the irregular distribution of these proteins reflects participation of different renal areas in facultative water reabsorption.
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Kidney360
December 2024
Department of Physics, P.O. Box 64, FI-00014 University of Helsinki, Finland.
Background: Structural analysis of soft biological tissues is conventionally done with destructive 2D histology. 3D information can be accessed with non-invasive imaging methods, such as X-ray micro-computed tomography (micro-CT). While attenuation-based X-ray imaging alone does not provide reasonable contrast with soft-tissue samples, the combination with contrast-enhancing staining has proven effective.
View Article and Find Full Text PDFIntroduction: Milan hypertensive strain (MHS) of rat represents as one of the ideal rat models to study the genetic form of hypertension associated with aberrant renal salt reabsorption. In contrast to Milan normotensive strain (MNS), MHS rats possess missense mutations in three adducin genes and develop hypertension at 3 months old due to upregulation of sodium-chloride cotransporter (NCC). At pre-hypertensive stage (23-25 days old), MHS rats show enhanced protein abundance of Na+-K+-2Cl- cotransporter (NKCC2) but retain blood pressure comparable to MNS probably through enhanced GFR and reduced NCC and α-subunit of epithelial sodium channel (α-ENaC) expressed in distal convoluted tubule (DCT) and collecting duct (CD).
View Article and Find Full Text PDFAm J Physiol Renal Physiol
November 2024
Department of Nephrology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Am J Physiol Renal Physiol
January 2025
Epithelial Systems Biology Laboratory, Systems Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States.
Epidermal growth factor (EGF) has important effects in the renal collecting duct to regulate salt and water transport. To identify elements of EGF-mediated signaling in the rat renal inner medullary collecting duct (IMCD), we carried out phosphoproteomic analysis. Biochemically isolated rat IMCD suspensions were treated with 1 µM of EGF or vehicle for 30 min.
View Article and Find Full Text PDFPLoS One
October 2024
Department of Anatomy, College of Graduate Studies, Midwestern University, Glendale, Arizona, United States of America.
The Arabian oryx (Oryx leucoryx) is an antelope that is considered a "vulnerable" species. Lack of knowledge on the structure of its reproductive organs hinders the development of strategies to improve its reproduction. There is only one report on the gross anatomy of the female reproductive organs.
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