The heterogeneity and the complexity of Emerson strain Chlorella pyrenoidosa chloroplastic DNA have been investigated by means of thermal denaturation and renaturation kinetics, and the results have been compared with those of the strain 211/8b of the same alga. The thermal denaturation properties are very close to those of the other strain: the Tm of 65 degrees C in 0.1 standard saline citrate, the maximal hyperchromicity of 41%, and the dispersion coefficent delta 2/3 of 6.65 degrees C. The first derivated curves of the melting profiles show also five components. Denatured chloroplastic DNA renatures rapidly. Two fractions are found; their kinetic complexities have been estimated: 1.5 times 10(7) daltons for the fast renaturing fraction; 2x 10(8) daltons for the low d (G + C) content of the chloroplastic DNA: 1.24 times 10(8) daltons). The unique nucleotide sequence is present in about 19 copies per chloroplastic genome. This report confirms the homogeneity of the chloroplastic genome of algae.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF00436330 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative analysis of the complete chloroplast genome of seven Wikstroemia taxa (Thymelaeaceae) provides insights into the genome structure and phylogenetic relationships.
Planta
January 2025
Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai, 200438, China.
New insights into the phylogeny of species in the family Thymelaeaceae and support of the recognition of D. genkwa and D. aurantiaca as species in the genus Wikstroemia are provided.
View Article and Find Full Text PDFThe complete chloroplast genome of 'Yunqie 9'.
Mitochondrial DNA B Resour
January 2025
School of Agriculture, Yunnan University, Kunming, China.
'Yunqie 9' was selected by the Horticultural Research Institute of Yunnan Academy of Agricultural Sciences based on the local environment of Yunnan Province. It is excellent in fruit quality and yield, but it is relatively weak in disease resistance. No information on complete chloroplast genome and position in the phylogeny of to restrict its genetic improvement.
View Article and Find Full Text PDF[Establishment and application of chloroplast genome database with the largest number of species in world].
Zhongguo Zhong Yao Za Zhi
December 2024
Experimental Research Center, China Academy of Chinese Medical Sciences Beijing 100700, China.
The chloroplast genome is an important tool for studying plant classification, evolution, and the heterologous production of secondary metabolites and protein drugs. With advancements in sequencing technology and reductions in sequencing costs, chloroplast genome data have rapidly accumulated. However, existing chloroplast genome databases suffer from issues such as incomplete data, inadequate management, and inconsistent, inaccurate information, posing significant challenges for the development and utilization of the chloroplast genome.
View Article and Find Full Text PDF[Establishment of a standard DSS library for identification of original plants of medicinal materials in Chinese Pharmacopoeia].
Zhongguo Zhong Yao Za Zhi
December 2024
Experimental Research Center,China Academy of Chinese Medical Sciences Beijing 100700, China.
With the development of molecular pharmacognosy, the advantages of DNA molecular markers in the identification of original plants of Chinese medicinal materials are becoming increasingly significant. To compensate for the limitations of existing markers in the quality supervision of Chinese medicinal materials, our team has independently designed a new molecular marker named DNA signature sequence(DSS). This marker is a nucleotide sequence that only appears in a specific taxonomic unit, with a length of 40 bp and high identification accuracy.
View Article and Find Full Text PDF[Mini-barcode combined with ITS2 for identification of bulk Artemisiae Scopariae Herba].
Zhongguo Zhong Yao Za Zhi
December 2024
State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine Tianjin 301617, China.
Artemisiae Scoporiae Herba is derived from Artemisia scoparia or A. capillaris. The accurate identification of the herbs, particularly when dealing with bulk samples, is critical for ensuring the quality and efficacy of the medicinal product.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!