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Detection of Hepatitis C Virus Infection from Patient Sera in Cell Culture Using Semi-Automated Image Analysis.

Viruses

November 2024

Department of Infectious Diseases, Molecular Virology, Section Virus-Host Interactions, Heidelberg University, 69120 Heidelberg, Germany.

The study of hepatitis C virus (HCV) replication in cell culture is mainly based on cloned viral isolates requiring adaptation for efficient replication in Huh7 hepatoma cells. The analysis of wild-type (WT) isolates was enabled by the expression of SEC14L2 and by inhibitors targeting deleterious host factors. Here, we aimed to optimize cell culture models to allow infection with HCV from patient sera.

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Heparanase 2 Modulation Inhibits HSV-2 Replication by Regulating Heparan Sulfate.

Viruses

November 2024

Department of Ophthalmology and Visual Sciences, College of Medicine, University of Illinois Chicago, Chicago, IL 60612, USA.

The host enzyme heparanase (HPSE) facilitates the release of herpes simplex virus type 2 (HSV-2) from target cells by cleaving the viral attachment receptor heparan sulfate (HS) from infected cell surfaces. HPSE 2, an isoform of HPSE, binds to but does not possess the enzymatic activity needed to cleave cell surface HS. Our study demonstrates that HSV-2 infection significantly elevates HPSE 2 protein levels, impacting two distinct stages of viral replication.

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The rapid integration of Internet of Things (IoT) systems in various sectors has escalated security risks due to sophisticated multilayer attacks that compromise multiple security layers and lead to significant data loss, personal information theft, financial losses etc. Existing research on multilayer IoT attacks exhibits gaps in real-world applicability, due to reliance on outdated datasets with a limited focus on adaptive, dynamic approaches to address multilayer vulnerabilities. Additionally, the complete reliance on automated processes without integrating human expertise in feature selection and weighting processes may affect the reliability of detection models.

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Flexible Piezoresistive Film Pressure Sensor Based on Double-Sided Microstructure Sensing Layer.

Sensors (Basel)

December 2024

Joint International Research Laboratory of Information Display and Visualization, School of Electronic Science and Engineering, Southeast University, Nanjing 210096, China.

Flexible thin-film pressure sensors have garnered significant attention due to their applications in industrial inspection and human-computer interactions. However, due to their ultra-thin structure, these sensors often exhibit lower performance, including a narrow pressure response range and low sensitivity, which constrains their further application. The most commonly used microstructure fabrication methods are challenging to apply to ultra-thin functional layers and may compromise the structural stability of the sensors.

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Amidst the backdrop of the profound synergy between navigation and visual perception, there is an urgent demand for accurate real-time vehicle positioning in urban environments. However, the existing global navigation satellite system (GNSS) algorithms based on Kalman filters fall short of precision. In response, we introduce an elastic filtering algorithm with visual perception for vehicle GNSS navigation and positioning.

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