The amplified fragment length polymorphism (AFLP) technique is a DNA technology that generates the so-called AFLP markers. These markers are genomic restriction fragments detected after two rounds of polymerase chain reaction (PCR) without prior knowledge of nucleotide sequence. Here we describe the first application of the AFLP technique in the rabbit. We have tested two primer combinations. The results obtained with the DNA from rabbits of different breeds justify the conclusion that AFLP analysis is an effective tool for genetic studies in the rabbit. In addition, we contribute to the linkage map of the rabbit by localizing two AFLP markers on rabbit linkage group VI (LG VI). For this purpose the progeny of a IIIVO/JU x [IIIVO/JU x AX/JU]F(1) backcross were genotyped for 12 AFLP markers and 3 LG VI classical markers [one coat color marker (e) and two biochemical markers (Es-1 and Est-2)]. AX/JU is a dietary cholesterol-susceptible (hyperresponding) inbred strain and IIIVO/JU is a dietary cholesterol resistant (hyporesponding) inbred strain. Moreover, it is possible to evoke dietary cholesterol-induced aorta atherosclerosis in a relatively short time period in AX/JU rabbits, in contrast to IIIVO/JU rabbits. A significant cosegregation was found between basal serum HDL cholesterol level (i.e., the level on a low-cholesterol, control diet) and an AFLP marker on LG VI. It is concluded that one or more genes of LG VI are regulating the basal serum HDL cholesterol level in rabbits. Thus the present study with rabbits clearly illustrates the value of AFLP markers for the construction of linkage maps and mapping of quantitative trait loci (QTL).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/jhered/92.4.322 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Reduced portal vein blood flow velocity in acute fatty liver of pregnancy.
Front Med (Lausanne)
December 2024
Department of Medical Ultrasound, Jinshan Hospital of Fudan University, Shanghai, China.
Purpose: Acute fatty liver of pregnancy (AFLP) is a severe complication that can occur in the third trimester or immediately postpartum, characterized by rapid hepatic failure. This study aims to explore the changes in portal vein blood flow velocity and liver function during pregnancy, which may assist in the early diagnosis and management of AFLP.
Methods: This longitudinal study was conducted at a tertiary healthcare center with participants recruited from routine antenatal check-ups.
Analyses of the Genetic Diversity and Population Structures of spp. Clinical Isolates from Paraíba, Brazil.
J Fungi (Basel)
December 2024
Laboratory of Mycology, Department of Pharmaceutical Sciences, Federal University of Paraiba (UFPB), João Pessoa 58051-900, Brazil.
Unlabelled: Sporotrichosis is a subcutaneous mycosis of global distribution, capable of affecting both humans and animals, and caused by species of the genus spp. This study aimed to evaluate the genetic diversity and mating type distribution of clinical isolates of human sporotrichosis in Paraíba, Brazil, to better understand the population structure, epidemiology, and diversification of this pathogen, as well as to explore possible transmission routes.
Methods: A total of 36 clinical isolates were morphologically identified, and clinical and demographic data were collected.
Diversity on a small scale - phylogeography of the locally endemic dwarf succulent genus Oophytum (Aizoaceae) in the Knersvlakte of South Africa.
Ann Bot
December 2024
Institute of Plant Science and Microbiology, University of Hamburg, Germany.
Background And Aims: Oophytum (Aizoaceae) is a locally endemic genus of the extremely fast evolving subfamily Ruschioideae and consists of only two formally accepted species (O. nanum and O. oviforme).
View Article and Find Full Text PDFArticle Synopsis
- Genotyping is the analysis of an organism's DNA to determine its genetic traits and has applications in fields like agriculture, biomedicine, and conservation.
- Genetic markers are categorized into random markers (like RFLP and AFLP) and functional markers (like SCoT and SRAP), with the latter being less utilized in animal science despite their advantages.
- This review provides an overview of the different genotyping markers available, their benefits and limitations, and emphasizes the need for more use of functional markers in genetic research.
Genetic Diversity and Population Structure of Endangered Orchid in Fragmented Habitat Using Fluorescent AFLP Markers.
Plants (Basel)
October 2024
Southwest Landscape Architecture Engineering Research Center of State Forestry and Grassland Administration, College of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming 650224, China.
Article Synopsis
- Genetic diversity is essential for a species' evolutionary potential and effective conservation strategies, particularly in fragmented habitats.
- A study on food-deceptive orchids in Shangrila County found significant genetic diversity, with 376 alleles identified and a high Nei's gene diversity of 0.339.
- Despite habitat fragmentation, the orchids maintained considerable genetic diversity, with only 8.75% of genetic variation occurring between populations, indicating significant gene flow within and between those populations.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!