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Dioxin detection based on immunoassay using a polyclonal antibody against octa-chlorinated dibenzo-p-dioxin (OCDD). | LitMetric
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Dioxin detection based on immunoassay using a polyclonal antibody against octa-chlorinated dibenzo-p-dioxin (OCDD).

M Shimomura Y Nomura K H Lee K Ikebukuro I Karube

Analyst

Research Center for Advanced Science and Technology, The University of Tokyo, Japan.

Published: August 2001

An enzyme-linked immunosorbent assay (ELISA) using a polyclonal antibody against octa-chlorinated dibenzo-p-dioxin (OCDD) is presented. This method is based on a competitive reaction between OCDD and OCDD-HRP (horseradish peroxidase) conjugate against the antibody, whereby OCDD-HRP is detected colorimetrically at 450 nm. The detection limit of OCDD was 0.78 pg mL(-1). Optimizing the reaction conditions of the assay, cross reactivities of some dioxins against the antibody are discussed.

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 Source
http://dx.doi.org/10.1039/b104258kDOI Listing

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