We performed nasal lavage (NAL) combined with induced sputum to determine exudative inflammation in the upper and lower airways in patients with chronic sinusitis and in controls. To monitor plasma exudation into the respiratory lumen and loss of size-selectivity of the mucosa, we determined the sample-to-serum ratio of albumin and alpha-2-macroglobulin, Qa1b and Qa2m, and the dilution independent Relative Coefficient of Excretion, RCE=Qa2m/Qa1b. To detect low protein levels in NAL and induced sputum we adapted an ELISA system for alpha-2-macroglobulin described by Out et al. [Clin. Chim. Acta, 165 (1987) 277-288], and modified this into a sensitive ELISA for albumin. Dithiothreitol, added to increase sputum solubility, did not interfere with the analysis, nor did N-ethylmaleimide, added to block dithiothreitol. In this study plasma exudation in induced sputum is significantly increased in patients with chronic sinusitis, compared to controls. Plasma exudation in NAL is also increased in patients, although not significant. The RCE in NAL and sputum is well-correlated in one of the three study visits. There is much variation in sample protein-levels partly due to differences in dilution and the heterogeneity of the studied population. Determination of plasma exudation together with RCE in NAL and induced sputum is a good, non-invasive way to quantify inflammation of airway mucosa.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1016/s0022-1759(01)00439-2 | DOI Listing |
Int J Surg Case Rep
January 2025
Cardiologist, School of Medicine, Urmia University of Medical Sciences, Urmia, West Azerbaijan, Iran. Electronic address:
Introduction And Importance: Diabetic foot ulcers, especially when complicated by cellulitis, pose a significant challenge in diabetes management, often leading to amputation. This case report highlights the successful treatment of a diabetic foot ulcer in an amputation candidate using a combination of negative pressure wound therapy and platelet-rich plasma injection, potentially reducing the risk of amputation in high-risk patients.
Case Presentation: A 62-year-old male with poorly controlled diabetes presented with a chronic diabetic foot ulcer and cellulitis.
Medicina (Kaunas)
December 2024
Faculty of Dental Medicine, "Dunarea de Jos" University, Al. I. Cuza Street 35, 800216 Galati, Romania.
: This study aimed to evaluate the role of A-PRF (advanced platelet-rich fibrin) in the enhancement of wound healing and protecting the periodontal health of mandibular second molars after the extraction of mandibular third molars. Additionally, the study assessed the levels of pro-inflammatory cytokines in the gingival crevicular fluid (GCF) of mandibular second molars as markers of inflammation. : Twenty-five systemically healthy adult patients with bilateral removal of impacted mandibular third molars were included.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
January 2025
Department of Biology, Indiana University, Bloomington, IN 47405.
Planta
December 2024
College of Resources, Hunan Agricultural University, Changsha, 410125, China.
Overexpression of OsSTP1 enhances the non-structural carbohydrate remobilization in the source, starch accumulation in grains, and the transportation of carbohydrates from source to sink during the filling stage. The sugar transporter protein (STP) is the best-characterized subfamily of the monosaccharide transporter (MST) family and plays critical roles in regulating plant stress tolerance, growth, and development. However, the role of STPs in regulating rice yield is poorly understood.
View Article and Find Full Text PDFJ Clin Periodontol
February 2025
Department of Oral Pathology and Conservative Dentistry, Periodontics, Faculty of Dentistry, Universidad de Los Andes, Santiago, Chile.
Aim: To characterize the gingival crevicular fluid (GCF) and plasma extracellular vesicles (EVs) and explore their proteomic cargo in healthy pregnant women compared to those with gestational diabetes mellitus (GDM) and periodontitis.
Methods: One-hundred and four pregnant women were recruited at 24-30 gestation weeks. GDM was diagnosed by an oral glucose tolerance test.
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!