AI Article Synopsis
- This paper presents an electrochemical biosensor designed to monitor free cholesterol levels using a multienzymatic system that integrates horseradish peroxidase and cholesterol oxidase on a pyrolitic graphite electrode.
- The study evaluates the sensor's efficiency, reproducibility, and stability based on voltammetric and amperometric data.
- These findings mark a significant step towards developing a mediator-free biosensor, which could have promising applications in the field of biosensors.
Article Abstract
This paper describes an electrochemical biosensor for free cholesterol monitoring. The sensor is a multienzymatic electrodic system in which horseradish peroxidase and cholesterol oxidase are simultaneously immobilized within a polymeric film, on the surface of a pyrolitic graphite electrode. From voltammetric and amperometric (flow-injection) data obtained, the efficiency, reproducibility and stability of the system are discussed. Results obtained, of interest for basic and applied biochemistry, represent a first step for construction of a mediator-free biosensor with potentialities for a successful application in the biosensor area.
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| http://dx.doi.org/10.1016/s0302-4598(01)00105-2 | DOI Listing |
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