The exact roles and abilities of the individual components of the lipopolysaccharide (LPS) receptor complex of proteins remain unclear. MD-2 is a molecule found in association with toll-like receptor 4. We produced recombinant human MD-2 to explore its LPS binding ability and role in the LPS receptor complex. MD-2 binds to highly purified rough LPS derived from Salmonella minnesota and Escherichia coli in five different assays; one assay yielded an apparent KD of 65 nm. MD-2 binding to LPS did not require LPS-binding proteins LBP and CD14; in fact LBP competed with MD-2 for LPS. MD-2 enhanced the biological activity of LPS in toll-like receptor 4-transfected Chinese hamster ovary cells but inhibited LPS activation of U373 astrocytoma cells and of monocytes in human whole blood. These data indicate that MD-2 is a genuine LPS-binding protein and strongly suggest that MD-2 could play a role in regulation of cellular activation by LPS depending on its local availability.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1074/jbc.M105228200 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of Mindfulness Therapy on Breast Cancer Patients with Depression or Anxiety: A Systematic Review and Meta-analysis.
Actas Esp Psiquiatr
March 2025
Department of Nursing, The Women's and Children's Hospital of Linping District Hangzhou, 311100 Hangzhou, Zhejiang, China.
Background: Breast cancer (BC) is the most common malignant tumor threatening women's health globally, with rising incidence rates and significant psychological impacts, particularly in China, where the prevalence of depressive and anxious mood disorders among BC patients is notably higher than the global average. To evaluate the effect of mindfulness therapy on anxiety and depressive symptoms in breast cancer patients, as psychological distress significantly affects their quality of life and often persists despite medical treatment.
Methods: A computerized search of Web of Science, PubMed, ScienceDirect, Cochrane Library, and Embase databases was conducted for randomized controlled trials involving the effects of positive thinking interventions on anxiety and depressive symptoms in breast cancer patients.
Comparing Nasal Splints and Quilting Sutures in Septoplasty: A Systematic Review and Meta-analysis.
Indian J Otolaryngol Head Neck Surg
February 2025
Department of Otolaryngology-Head and Neck Surgery, King Abdulaziz University Hospital, King Saud University, King Khaled Road, Riyadh, 11223 Saudi Arabia.
Background: Nasal packing post-septoplasty often leads to discomfort, breathing difficulties, and elevated infection risk. Alternative methods, such as intranasal splinting and quilting sutures, have been proposed to mitigate these issues. This study evaluates their effectiveness in reducing postoperative pain and complications.
View Article and Find Full Text PDFBackground: Medial meniscal extrusion (MME) is both a contributor to and a consequence of medial knee osteoarthritis (OA), with each condition exacerbating the other.High tibial osteotomy (HTO) realigns varus to valgus to alleviate medial compartment stress.This study conducted a systematic review and meta-analysis to evaluate alterations in medial meniscus extrusion values and clinical outcomes following high tibial osteotomy (HTO).
View Article and Find Full Text PDFEfficacy and safety of Chinese classical prescriptions for dilated cardiomyopathy: a systematic review and Bayesian network meta-analysis.
Syst Rev
March 2025
Department of Cardiology, Guang'Anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China.
Background: Chinese classical prescriptions (CCPs) are commonly utilized in China as an adjuvant treatment for dilated cardiomyopathy (DCM). Nevertheless, there was insufficient systematic evidence data to show the advantages of CCPs plus current conventional therapy (CT) against DCM. This network meta-analysis (NMA) sought to evaluate and prioritize the six different CCP types' respective efficacies for DCM.
View Article and Find Full Text PDFComparative efficacy of cast immobilization versus removable braces in patients with ankle fractures: a systematic review and meta-analysis.
BMC Musculoskelet Disord
March 2025
Department of Orthopedic Surgery, Hai'an People's Hospital, Zhongba Road 17, Hai'an, Nantong, Jiangsu, 226600, People's Republic of China.
Background: An effective and appropriate method to support the ankle joint optimally is particularly important during the healing phase of ankle fractures. The purpose of this review was to assess the functional outcomes, ankle-related quality of life, and associated complications of cast immobilization versus removable braces for the treatment of adult ankle fractures.
Methods: Studies comparing cast immobilization and removable braces after ankle fracture were included by systematic searches of PubMed/MEDLINE, Web of Science, Scopus, and EMBASE databases according to PRISMA (Preferred Reporting Items for Systematic Evaluation and Meta-Analysis Statements) guidelines.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!