AI Article Synopsis
- The study aimed to explore how propofol and ketamine affect TNF-alpha gene expression in immune cells, focusing on their direct interactions.
- Propofol was found to significantly increase TNF-alpha mRNA and protein levels, while ketamine reduced these levels in cultured blood stimulated with E. coli endotoxin.
- The findings indicate that propofol enhances TNF-alpha gene transcription at clinically relevant concentrations, whereas ketamine's inhibitory effects require higher doses.
Article Abstract
Aim: Anesthetic agents have direct and indirect effects on immunocompetent cells but the molecular mechanisms of direct interactions are largely unknown. Therefore, the effect of propofol and ketamine on TNF-alpha gene expression was studied in cultured blood from healthy volunteers.
Methods: TNF-alpha was measured in blood cultured in the absence or presence of propofol or ketamine after stimulation with E. coli endotoxin. TNF-alpha concentrations were correlated with the expression of TNF-alpha mRNA in mononuclear cells. The half-life of TNF-alpha mRNA was assessed after addition of actinomycin D, an inhibitor of DNA-directed RNA synthesis.
Results: Propofol increased TNF-alpha mRNA (+42%) and protein (+44%) response while the addition of ketamine attenuated the TNF-alpha mRNA (-31%) and protein (-53%) response. The half-life of TNF-alpha mRNA was unaffected by the anesthetics.
Conclusion: These data suggest that propofol and ketamine have opposite effects on transcription of the TNF-alpha gene. While the effects of propofol were observed with concentrations corresponding to an induction bolus, the effects of ketamine were restricted to higher concentrations.
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| http://dx.doi.org/10.1007/s001010100149 | DOI Listing |
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