A novel lateral flow card (TS-Card pork) test was developed for the serological detection of Trichinella infected pigs. Based on extensive studies performed in Romania during 1999-2000 this test proved to be highly specific sensitive, rapid (3-12 minutes) and easy to use (no need for laboratory facilities). It can be used both for the detection of Trichinella infection in carcasses and for epizooliological studies using a variety of samples including whole or dried blood, serum, or tissue fluids. The TS-Card pork test, used as a screening test, can be the foundation of an on-farm or field based inspection system to significantly improve food safety in countries with a high prevalence of Trichinella in pigs or other food animal species. The results presented are also promising for application of the test in an on-line laboratory based inspection system since the speed of the test allows sufficient time to rail out suspected hog carcasses during the slaughter process.
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http://dx.doi.org/10.1051/parasite/200108s2240 | DOI Listing |
Vet Parasitol Reg Stud Reports
January 2025
Minnesota Department of Natural Resources, Wildlife Health Program, 5463 West Broadway, Forest Lake, MN 55025, USA.
During a survey for other parasitic infections, tongue and limb muscles of wild bobcats (Lynx rufus) were examined microscopically from a sample of 190 Minnesota bobcats. One batch (n = 50) was studied in 2015, yielding no cases of Trichinella when examined only with hematoxylin and eosin (HE) staining of <25 mg of tissue. In a 2024 cohort (n = 140), this method was paired with a compression of ∼5 g of tissue.
View Article and Find Full Text PDFFood Waterborne Parasitol
March 2025
Unit of Foodborne and Neglected Parasitic Diseases, Department of Infectious Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
is a zoonotic nematode parasite of worldwide distribution. It is present in Europe with important foci, particularly in Eastern countries and Spain. This species is generally associated with a domestic cycle that involves primarily pigs.
View Article and Find Full Text PDFSci Rep
December 2024
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
The cytokine homologs, particularly transforming growth factor (TGF)-β, is a crucial immunomodulatory molecule and involved in growth and developmental processes in several helminths. In this study, the basic properties and functions of T. spiralis TGF-β homolog 2 (TsTGH2) were characterized using bioinformatics and molecular biology approaches.
View Article and Find Full Text PDFParasitol Int
December 2024
Department of Veterinary Pathobiology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX, USA. Electronic address:
Zoonotic parasitic infections can affect both animal and human health, while also having food safety and economic implications. These pathogens can also pose occupational risks for certain groups, including veterinary professionals, animal handlers, and butchers. This study was conducted in the district of Swabi located in the Khyber Pakhtunkhwa Province of northwestern Pakistan.
View Article and Find Full Text PDFJ Parasitol
December 2024
Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, Pennsylvania 16802.
During a survey for Sarcocystis infections in Pennsylvania in wild canids, muscles from the tongue and limb were examined microscopically for sarcocysts. Between 9 February 2024 and 11 February 2024, muscle samples were collected from 76 coyotes, 46 gray foxes, and 21 red foxes from Pennsylvania hunter harvested animals. Around 5 g of muscle was examined microscopically by compression between a glass slide and coverslip.
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