AI Article Synopsis
- A study involved 26 Yemeni patients with visceral leishmaniasis (VL), assessing the efficacy of a C-ELISA test compared to traditional microscopy for diagnosing the disease.
- The C-ELISA test showed higher sensitivity, identifying additional positive cases missed by microscopy, with 82.3% of patients testing positive on both methods.
- Among the treated patients, 76.3% responded to sodium antimony gluconate, with the C-ELISA effectively monitoring treatment success by showing a decrease in antibody inhibition in drug-responsive patients.
Article Abstract
Twenty six Yemeni patients with clinically visceral leishmaniasis (VL) and laboratory abnormalities were subjected to L. donovani species-specific monoclonal antibody (D2) and a competitive enzyme-linked immunosorbent assay (C-ELISA). The results were compared with those obtained by microscopy Giemsa stained tissue smears of bone marrow or splenic aspiration. Of the patients, 51 (82.3%) were positive and 3 (4.8%) were negative by both microscopy and C-ELISA. An additional 8 (12.9%) who were negative by microscopy were positive by C-ELISA. 59 patients were treated with sodium antimony gluconate, 45 (76.3%) were drug responsive and 14 (23.7%) were drug resistant. The C-ELISA of 17 patients before and after chemotherapy showed a significant decrease in percent inhibition of monoclonal antibodies D2 in drug responsive patients. In drug-unresponsive patients the percent inhibition of D2 was unchanged or slightly increased. The results indicate that C- ELISA is more sensitive and specific than microscopy, especially for early diagnosis of VL and to evaluate the success of drug treatment.
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