Modification of collagen film by certain chemical agents.

Arch Immunol Ther Exp (Warsz)

Department of Transplantology and Central Tissue Bank, Center of Biostructure Research, Warsaw Medical University, Poland.

Published: December 2001

AI Article Synopsis

  • The study examined how trypsin, hydrochloric acid (HCl), and EDTA affect the structure and free carboxyl group content of type I bovine collagen films.
  • Trypsin and HCl treatment led to film delamination and the emergence of low-density spots, indicating structural changes.
  • In contrast, EDTA did not produce any noticeable morphological changes, but trypsin significantly increased the concentration of free carboxyl groups by ten times compared to the control.

Article Abstract

Morphological changes and the content of free carboxyl groups in bovine collagen (type I) film under the influence of trypsin, hydrochloric acid (HCl) and ethylenediaminetetraacetic acid (EDTA) were studied. Incubation with trypsin and HCl was found to cause some delamination of the film and the appearance of some low-density spots. Incubation with EDTA did not cause any morphological changes. A high concentration of free carboxyl groups (10-fold higher than in control) was seen after incubation with trypsin.

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