The present study was conducted to compare the incidences of renal tumors in Wistar (W), Fischer (F) and F1 rats (WF: female Wistar rats x male Fischer rats; FW: female Fischer rats x male Wistar rats) induced by N-ethyl-N-hydroxyethylnitrosamine (EHEN). Levels of 8-OHdG in renal DNA were also investigated in Wistar and Fischer rats. After 2000 ppm of EHEN was administered orally for 2 weeks, the animals were fed basal diet until week 32. Wistar males and females demonstrated significantly higher sensitivity regarding induction of renal lesions, while both WF and FW rats had similar incidences, generally intermediate between those for the two parent strains. The formation of 8-OHdG was maximal 60-180 min after an intraperitoneal dose of 750 mg/kg to Wistar and Fischer rats, which correlates with the increase tending to the incidence of renal tumors in male and female Wistar and Fischer rats. The results suggest that EHEN induction of renal tumors is related to oxygen radical damage and that the genes in the Wistar strain responsible for the sensitivity are not inherited in a sex-dependent fashion, despite the male being more susceptible.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0304-3835(01)00608-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ageing impairs endothelium-dependent vasodilatation and alters redox signalling in diaphragm arterioles from male and female Fischer-344 rats.
J Physiol
March 2025
Department of Kinesiology, Kansas State University, Manhattan, Kansas, USA.
Diaphragm hyperaemia and regional blood flow distribution are impaired with ageing, potentially consequent to altered vascular structure and/or diminished vasomotor function. Evidence from locomotory skeletal muscle suggests that age-related diaphragm vasomotor dysfunction may be related to a blunted endothelium-mediated vasodilatation, decreased nitric oxide (NO) bioavailability and/or augmented reactive oxygen species (ROS) generation. We hypothesized that, in the medial costal diaphragm with old age, there would be fewer feed arteries (FAs) and impaired vasomotor function, via endothelium-specific mechanisms, in first-order (1A) arterioles.
View Article and Find Full Text PDFRest-to-work and work-to-rest transients of interstitial PO in spinotrapezius muscle of young and old male rats.
Physiol Rep
March 2025
Song Biotechnologies LLC, Baltimore, Maryland, USA.
Muscle function declines with age. Since the primary energy source for contraction is aerobic, this study investigated age-related changes in muscle oxygenation dynamics to: characterize PO transients during rest-work transitions, identify age-specific differences in oxygen delivery/utilization balance, and examine the relationship between interstitial and arterial oxygen tension (PO). Interstitial PO was measured with a high-resolution stroboscopic phosphorescence quenching technique to map intra-contractile dynamics during changes in muscle activity-rest-to-work (RtW) and work-to-rest (WtR) in rats aged three (young) and 23 (old) months.
View Article and Find Full Text PDFBackground: Pulmonary hypertension (PH) results in increased right ventricular (RV) afterload, leading to RV dysfunction and failure. The mechanisms underlying maladaptive RV remodeling are poorly understood. In this study, we investigated the multiscale and mechanistic nature of RV free-wall (RVFW) biomechanical remodeling and its correlations with RV function adaptations.
View Article and Find Full Text PDFWell-Preserved Urinary Bladder Anatomy in Rats After Minimally Invasive Surgery.
Biomedicines
January 2025
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, I-80137 Napoli, Italy.
The setup of experimental protocols able to preserve the anatomical integrity also in terms of organ microarchitecture is mandatory to ensure result translatability. Also, the maintenance of structural integrity perfectly aligns with the refinement implementation aiming to reduce procedure severity, a key issue in animal studies deemed compulsory from both ethical and legal standpoints. Here we report a detailed description of all peri-operative and post-operative care and clinical evaluation in a surgical rat model to test the efficacy of a catheter functionalized by a peptide coating with antimicrobial and antibiofilm properties, whose efficacy was previously tested in vitro.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!