A feline embryo cell line was treated in vitro with various levels of 7,12-dimethylbenz[a]anthracene (DMBA) or dimethyl sulfoxide (control). Repeat treatment of DMBA only induced in vitro transformation of feline embryo cells following clonal growth and selection. The morphologically altered cells formed large cell aggregates and grew in this aggregate form when suspended in liquid growth medium above an agar base, formed colonies in soft agar, and grew to high saturation densities. However, no progressively growing tumors were produced when cells were inoculated into nude athymic mice. The transformed lines were negative for feline oncornavirus-associated cell membrane antigen (FOCMA).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/ijc.2910240311 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Glycosylation in Canine Placentas.
Reprod Domest Anim
December 2024
Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada (LHYEDEC), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata (FCV, UNLP), La Plata, Argentina.
This article describes the carbohydrate composition of early and mature placentas from bitches, detected by lectin histochemistry. Formalin-fixed placental samples from 11 mixed-breed bitches have been assigned to the 'early' or the 'mature' placenta group, processed by the routine histological technique and labelled with a panel of 14 biotinylated lectins. The glycan distribution was almost completely preserved over pregnancy.
View Article and Find Full Text PDFNovel electroporation microchip with field constriction enhances transfection efficiency and survival rates of feline embryos.
Sci Rep
November 2024
Department of Obstetrics, Gynaecology, and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
This study introduces a low-voltage electroporation microchip designed for transfection in cat embryos, featuring real-time impedance monitoring. The microchip uses a field constriction strategy, which localises the electric field to the membrane region in contact with the micro-orifice, enhancing electroporation efficiency while minimising damage. Embryos were positioned on the orifice, and a series of voltage pulses (10, 15, and 20 V) were applied.
View Article and Find Full Text PDFApproaching the sterile womb theory in dogs and cats: A multi-technique investigation.
Theriogenology
February 2025
Department of Veterinary Sciences, University of Turin, 10095, Grugliasco, Italy.
Article Synopsis
- - The study explored bacterial presence in the womb of dogs and cats using various methods like gene sequencing and culture analysis, with samples taken from pregnant animals undergoing surgery.
- - Bacterial sequences were found in all fetal samples, but contamination from controls highlighted the complexity of understanding bacterial diversity and composition in these low-biomass samples.
- - Key findings revealed Moraxella spp. and Burkholderia cepacia in canine and feline placentas, respectively, but the presence of bacterial DNA doesn't imply a functioning fetal microbiome, suggesting that this DNA likely comes from the mother rather than a resident fetus.
Feline cumulus cells and oocytes show massive accumulation of lipid droplets and upregulation of PLIN2 expression after in vitro maturation.
Theriogenology
January 2025
Poznan University of Life Sciences, Faculty of Veterinary Medicine and Animal Science, Department of Genetics and Animal Breeding, Wolynska 33, 60-637, Poznan, Poland. Electronic address:
This study investigates lipid content and expression of genes involved in lipid metabolism in feline cumulus cells and oocytes before and after in vitro maturation (IVM). Domestic cats represent valuable models in reproductive research, yet the efficiency of in vitro embryo production remains suboptimal, with contributing factors still under investigation. We characterized lipid droplets (LDs) in oocytes collected from adult queens, both before and after IVM, using confocal microscopy with Bodipy 493/503 staining.
View Article and Find Full Text PDFEnhancing felid conservation: Exploring the impact of in vitro culture media on domestic cat blastocyst production.
Reprod Domest Anim
October 2024
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Complutense University of Madrid, Madrid, Spain.
Article Synopsis
- - This study examined ways to improve assisted reproductive techniques for conserving wild cats, using domestic cats as a reference.
- - Researchers tested three types of in vitro culture media to see how they influenced the formation of blastocysts, finding similar success rates of over 16%.
- - The study shows promise for these reproductive methods but suggests more research is needed to assess embryonic quality and enhance protocols for conserving wild felids.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!