AI Article Synopsis
- EGF treatment enhances NHE2-mediated Na(+) absorption and increases NHE2 mRNA levels in the intestines of young rats.
- EGF does not affect renal NHE2 or intestinal/renal NHE3 mRNA levels, indicating a specific action on NHE2.
- The stimulation of NHE2 activity by EGF involves gene transcription, as shown by increased promoter activity and the effect of transcription inhibitors in cultured intestinal cells.
Article Abstract
Epidermal growth factor (EGF) is involved in acute regulation of Na(+)/H(+) exchangers (NHEs), but the effect of chronic EGF administration on NHE gene expression is unknown. The present studies showed that EGF treatment increased NHE2-mediated intestinal brush-border membrane vesicle Na(+) absorption and NHE2 mRNA abundance by nearly twofold in 19-day-old rats. However, no changes were observed in renal NHE2 mRNA or intestinal and renal NHE3 mRNA abundance. To understand the mechanism of this regulation, we developed the rat intestinal epithelial (RIE) cell as an in vitro model to study the effect of EGF on NHE2 gene expression. EGF increased functional NHE2 activity and mRNA abundance in cultured RIE cells, and this stimulation could be blocked by actinomycin D (a transcriptional inhibitor). Additionally, NHE2 promoter reporter gene assays in transiently transfected RIE cells showed an almost twofold increase in promoter activity after EGF treatment. We conclude that rat NHE2 activity can be stimulated by chronic EGF treatment and that this response is at least partially mediated by gene transcription.
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| http://dx.doi.org/10.1152/ajpcell.2001.281.2.C504 | DOI Listing |
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