Plasma proteins of 139 healthy adult birds of prey from 10 species were separated by electrophoresis to characterize and document normal reference ranges and species-specific electrophoretic patternsand to evaluate the value of this technique for health screening, disease diagnosis, and prognostic indication. Species studied included bald eagle (Haliaeetus leucocephalus), red-tailed hawk (Buteo jamaicensis), barn owl (Tyto alba), great horned owl (Bubo virginianus), turkey vulture (Cathartes aura), Harris' hawk (Parabuteo unicinctus), Stellar's sea eagle (Haliaeetus pelagicus), barred owl (Strix varia), screech owl (Otus asio), and black vulture (Coragyps atratus). Several clinical cases show the diagnostic/therapeutic value of protein electrophoresis in raptors. This study establishes species-specific reference ranges for several birds of prey and discusses the benefit of electrophoresis as a diagnostic technique in health screens, as a diagnostic aid in conjunction with other tests, and as a prognostic indicator in clinical evaluation of raptors.
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http://dx.doi.org/10.1638/1042-7260(2000)031[0497:PEAADA]2.0.CO;2 | DOI Listing |
Background And Objective: Serum protein electrophoresis (SPEP) plays a critical role in diagnosing diseases associated with M-proteins. However, its clinical application is limited by a heavy reliance on experienced experts.
Methods: A dataset comprising 85,026 SPEP outcomes was utilized to develop artificial intelligence diagnostic models for the classification and localization of M-proteins.
Plant Dis
January 2025
Cornell University, Ithaca, New York, United States;
Azerbaijan is major producer of fruit crops, such as pome and stone fruits, in the Caspian Sea and Caucasus Mountains areas (FAO Stat, 2022). No information is available on the occurrence of apple chlorotic leaf spot virus (ACLSV, genus Trichovirus, family Betaflexiviridae) in the country. Therefore, the main fruit tree production areas in Azerbaijan were surveyed for ACLSV during the 2017-2019 growing seasons by DAS-ELISA using ACLSV reagents (Neogen - Scotland, UK) (Clark and Adams 1977).
View Article and Find Full Text PDFInvest Ophthalmol Vis Sci
January 2025
Affiliated Eye Hospital of Nanchang University, Jiangxi Research Institute of Ophthalmology and Visual Science, Jiangxi Provincial Key Laboratory for Ophthalmology, Jiangxi Clinical Research Center for Ophthalmic Disease, Nanchang, China.
Purpose: This study aimed to investigate the role of SIRT4 in retinal protection, specifically its ability to mitigate excitotoxic damage to Müller glial cells through the regulation of mitochondrial dynamics and glutamate transporters (GLASTs).
Methods: A model of retinal excitatory neurotoxicity was established in mice. Proteins related to mitochondrial dynamics, GLAST, and SIRT4 were analyzed on days 0, 1, 3, and 5 following toxic injury.
Invest Ophthalmol Vis Sci
January 2025
School of Graduate, Dalian Medical University, Dalian City, China.
Purpose: To investigate the effect of Ca2+/calmodulin-dependent protein kinase II (CAMKII) δ subtypes (CAMK2D) on sodium iodate (NaIO3)-induced retinal degeneration in mice.
Methods: Bioinformatics analysis and Western blot experiments were used to screen the significantly differentially expressed genes in age-related macular degeneration (AMD) disease. CAMK2D knockdown and overexpression models were constructed by lentivirus (LV) infection of adult retinal pigment epithelial cell line-19 (ARPE-19) cells in vitro.
Urol Res Pract
January 2025
Department of Pediatric Surgery, Zonguldak Bulent Ecevit University, Faculty of Medicine, Zonguldak, Türkiye.
Objective: Bladder tissue models have been developed using smooth muscle cells (SMCs) on various scaffolds to mimic bladder morphology and physiology. This study investigates the effects of co-culturing fetal and adult SMCs on growth properties and protein profiles to understand cellular interactions and population kinetics.
Methods: Bladder tissue samples from 10 adult and 10 fetal New Zealand rabbits were divided into 5 groups: adult SMCs (A), fetal SMCs (F), 50%A+50%F (A+F), 75%A+25%F (3A+F), and 25%A+75%F (A+3F).
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