A cross-sectional study on bionomics of malaria was carried out in Kamlek village, Snuol rubber plantation, Kratie Province where the population had a high prevalence of malaria. Mosquitos were collected monthly between April to September 1998 from 18.00 hr to 6.00 hr by human bait and animal bait. Survey for larva breeding places was also performed. A total of 13 species of Anopheline mosquitos was collected, manly Anopheles philipinensis, An. aconitus, An. annularis, Ain. barbirostris, An. hyrcanus gr, An. jamesii, An. kawari, An. tesselatus, An. umbrosus and An. vagus. They were in various densities with the latter species being the most abundance. Among the vector species. An. maculatus was the most commonly collected with a density of 0.55 per man per night and peak biting was between 20.00 hr and 21.00 hr. The density of An. minimus and An. dirus were 0.11 and 0.74 per man per night with the peak biting time 24.00-01.00 hr and 20.00-21.00 hr respectively. Observation of host preference and feeding habit revealed An. maculatus to be exophagic, anthropophilic and 20 endophilic while An. minimus was both endophilic and exophagic and anthropophilic. An. dirus was endophilic and anthropophilic, these three species were not found positive for malaria infection. However, due to the limited time of survey and an exceptionally low rainfall in the rainy season, an inadequate sample was obtained for analysis. Therefore further investigation is required for at least a year for more information.
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J Genet Eng Biotechnol
March 2025
National Institute of Agricultural Research (INRA), Regional Center of Agricultural Research Rabat, Biotechnology Unit, Rabat 10000 Morocco.
Thymus satureioides is an endemic and medicinal plant of Morocco, widely distributed in the arid and semiarid habitats. Communally used in traditional medicine. In the current study, twelve Inter-Simple Sequence Repeats (ISSR) primers combined with 11 agro-morphological traits were applied to evaluate 60 accessions of T.
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March 2025
Department of Ecology, University of Chicago, Chicago, Illinois 60637, USA.
Understanding how the auxin hormone signaling pathway components come together to orchestrate cellular responses is key to engineering the growth and development of maize. Although a variety of techniques exist to measure auxin activities in plants, many are time- and resource-intensive or do not easily allow for high-throughput quantitative measurement of component libraries. The AuxInYeast system is a synthetic biology tool that facilitates complex biochemical analysis of the auxin hormone signaling pathway from essentially any plant.
View Article and Find Full Text PDFCold Spring Harb Protoc
March 2025
Department of Biology, Whitman College, Walla Walla, Washington 99362, USA
The AuxInYeast system is a synthetic biology tool that facilitates complex biochemical analysis of the plant auxin hormone signaling pathway. As a plant synthetic biology chassis, yeast offers rapid growth, well-established genetic and biochemical tools, and core eukaryotic cellular machinery compatible with heterologous plant gene expression. The AuxInYeast system for maize consists of yeast cells containing the minimal necessary set of plant auxin signaling parts: a receptor (ZmTIR1/AFB), repressor (ZmIAA), corepressor (REL2), transcription factor (ZmARF), and auxin response -element (auxRE).
View Article and Find Full Text PDFCold Spring Harb Protoc
March 2025
Department of Biology, Whitman College, Walla Walla, Washington 99362, USA
Synthetic biology approaches merge the tenets of engineering with established biological techniques to answer fundamental questions about living systems and to engineer biological forms and functions. Following the engineering principle of design-build-test-iterate, this review serves as a guide to applying synthetic principles and approaches in maize. We outline strategies for (1) choosing the optimal model organism to serve as a heterologous chassis for maize signaling pathways, (2) designing and building biological parts and devices to express pathway components, (3) choosing an analytical technique to measure pathway function, and (4) optimizing and troubleshooting the designed system.
View Article and Find Full Text PDFGen Comp Endocrinol
March 2025
Department of Biological Sciences, Texas Tech University, Box 43131, Lubbock, TX 79409, USA. Electronic address:
Weddell seal (Leptonychotes weddellii) females lose substantial body mass across an intensive, nutritionally restricted lactation period and then must rapidly recover mass during the short Antarctic summer. In this study, we examined endocrine dynamics associated with mass loss across lactation and subsequent realimentation in Weddell seals, comparing patterns between seals that recently gave birth and demographically similar non-reproductive females (skip females) in McMurdo Sound, Antarctica. Postpartum seals near weaning (∼35 days postpartum, n = 64) and skip females (n = 32) were handled during early austral summer (November/December) and rehandled in late summer (January/February).
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