Listeria monocytogenes releases its antigens during the exponential phase. Electrosyneresis on agarose allows detection of these antigens in the supernatant of broth culture following a 3 h incubation of the strains. This serotyping method seems to be easy, rapid and reproductible. The authors have investigated 83 strains of different origines and find no difference with agglutination. However, during the stationary phase, L. monocytogenes releases other antigens which gave precipitation lines with all commercial sera tested.
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