Recombinant mouse 18 kDa peripheral-type benzodiazepine receptor (PBR) protein was overexpressed in Escherichia coli and isolated using a His. Bind metal chelation resin. Recombinant PBR protein was purified with sodium dodecyl sulfate and reincorporated into liposomes using Bio-Beads SM2 as a detergent removing agent. Negative staining of the reconstituted PBR samples, examined by electron microscopy, showed the formation of proteoliposomes. Freeze-fracture of these proteoliposomes revealed the presence of transmembranous particles of an average size of 3.5 +/- 0.25 nm, consistent with the presence of a monomeric form of the recombinant PBR protein. The reconstituted protein exhibited the ability to bind both the PBR drug ligand isoquinoline carboxamide PK 11195 and cholesterol with nanomolar affinities. These data suggest that a PBR monomer is the minimal functional unit, binding drug ligands and cholesterol.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1006/bbrc.2001.4975 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Preparative Isolation of N-glycans from Natural Sources Mediated by a Deglycosylating Heterogeneous Biocatalyst in Flow.
ChemSusChem
January 2025
CIC biomaGUNE, Heterogeneous Biocatalysis, Paseo Miramon 182, 20009, San Sebastian, SPAIN.
EEfficient methods for isolating N-glycans are essential to understanding the functions and characteristics of the entire N-glycome. Enzymatic release using PNGaseF is the most effective approach for releasing mammalian N-glycans for analytical purposes. However, the use of PNGaseF for preparative N-glycan isolation is precluded due to the enzyme's cost and limited stability.
View Article and Find Full Text PDFGlucagon-Like Peptide 1 Receptor Agonists and Chronic Lower Respiratory Disease Among Type 2 Diabetes Patients: Replication and Reliability Assessment Across a Research Network.
Pharmacoepidemiol Drug Saf
January 2025
Observational Health Data Science and Informatics, New York, New York, USA.
Introduction: The aim of this study is to use observational methods to evaluate reliability of evidence generated by a study of the effect of glucagon-like peptide 1 receptor agonists (GLP-1RA) on chronic lower respiratory disease (CLRD) outcomes among Type-2 diabetes mellitus (T2DM) patients.
Research Design And Methods: We independently reproduced a study comparing effects of GLP-1RA versus dipeptidyl peptidase-4 inhibitors (DPP4-i) on CLRD outcomes among patients with T2DM and prior CLRD. We reproduced inputs and outputs using the original study data (national administrative claims) and evaluated the robustness of results in comparison to alternate design/analysis decisions.
Photobioreactor systems for mitigating ammonia and carbon dioxide from a broiler house.
J Environ Manage
December 2024
Biosystems Engineering, Faculty of Agriculture, Bursa Uludag University, Gorukle, 16240, Bursa, Turkey; Biosystems Engineering, Faculty of Engineering-Architecture, Yozgat Bozok University, Yozgat, Turkey. Electronic address:
This study investigated the effectiveness of photobioreactor (PBR) systems in reducing air pollutants emitted from broiler houses. It focused on two microalgae species and one cyanobacteria grown under different media conditions and investigated their ability to mitigate ammonia (NH) and carbon dioxide (CO) in the exhaust air of a broiler house. Ankistrodesmus sp.
View Article and Find Full Text PDFRecent progress in therapeutic applications of fluorinated five-membered heterocycles and their benzo-fused systems.
RSC Adv
October 2024
Department of Chemistry, Faculty of Science, Cairo University Giza 12613 Egypt (+202) 35727556.
Article Synopsis
- * Over 20% of marketed medications contain fluorine, with nearly 300 approved fluorine-containing drugs, highlighting their significance in pharmaceuticals.
- * This review focuses on the therapeutic potential of five-membered heterocycles and their fused systems, detailing various activities like antiviral, anti-inflammatory, and more, often showing effectiveness comparable to standard reference drugs.
Safety and immunogenicity of a modified mRNA-lipid nanoparticle vaccine candidate against COVID-19: Results from a phase 1, dose-escalation study.
Hum Vaccin Immunother
December 2024
GSK, Wavre, Belgium.
This phase 1, open-label, dose-escalation, multi-center study (NCT05477186) assessed the safety and immunogenicity of a booster dose of an mRNA COVID-19 vaccine (CV0501) encoding the SARS-CoV-2 Omicron BA.1 spike protein. Participants aged ≥ 18 years previously vaccinated with ≥ 2 doses of an mRNA COVID-19 vaccine received CV0501 doses ranging from 12 to 200 μg.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!