It was found that, depending on their frequency, mechanical vibrations (MVs) can either stimulate (4 Hz) or inhibit (50 Hz) the growth and the division of the lon mutant of Escherichia coli K-12. Similar effects were observed when the MV-treated nutrient medium was inoculated with untreated mutant cells. MVs enhanced the motility of mutant cells and the fragmentation of filament cells always present in the populations of lon mutants.
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PLoS One
January 2025
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Tigecycline (Tgc), a third-generation tetracycline is found as the last line of defense against multi-drug resistant bacteria. Recent increased rate of resistance to tgc, a human-restricted agent among animal bacteria poses a significant global health challenge. Overuse of first generation tetracyclines (Tet) and phenicols in animals have been suggested to be associated with Tgc resistance development.
View Article and Find Full Text PDFPlant J
December 2024
Department of Biosciences, Rice University, Houston, Texas, 77005, USA.
Peroxisomes house diverse metabolic pathways that are essential for plant and animal survival, including enzymes that produce or inactivate toxic byproducts. Despite the importance of peroxisomes and their collaborations with other organelles, the mechanisms that trigger or prevent peroxisome turnover and the cellular impacts of impaired peroxisomes are incompletely understood. When Arabidopsis thaliana LON2, a peroxisomal protein with chaperone and protease capacity, is disrupted, metabolic dysfunction and protein instability in peroxisomes ensue.
View Article and Find Full Text PDFFront Microbiol
October 2024
Department of Bacteriology, Animal and Plant Health Agency, Addlestone, United Kingdom.
Disinfectants are essential for biosecurity, preventing the persistence and spread of zoonotic pathogens on farms and subsequent human infections. In this study, transcriptomics and genomics were utilised to assess the effect of disinfectant exposure on pathogenic . The exposure of O157:H7 to sub-optimal concentrations of commonly used farm disinfectants elicited changes in both the transcriptome and genome.
View Article and Find Full Text PDFGene
January 2025
Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China. Electronic address:
Cell cycle adaptability assists bacteria in response to adverse stress. The effect of oxidative stress on replication initiation in Escherichia coli remains unclear. This work examined the impact of exogenous oxidant and genetic mutation-mediated oxidative stress on replication initiation.
View Article and Find Full Text PDFGene
January 2025
Center for Energy Conservation and Emission Reduction in Fermentation Industry in Inner Mongolia, Hohhot 010051, Inner Mongolia, China; Engineering Research Center of Inner Mongolia for Green Manufacturing in Bio-fermentation Industry, Hohhot 010051, Inner Mongolia, China; Specialized Technology Research and Pilot Public Service Platform for Biological Fermentation in Inner Mongolia, Hohhot 010051, Inner Mongolia, China; College of Chemical Engineering, Inner Mongolia University of Technology, Hohhot 010051, Inner Mongolia, China. Electronic address:
Objective: Most protein secretion systems are found in gram-negative bacteria, but the mechanism of endoglucanase (BcsZ) secretion in Escherichia coli (E. coli) remains unclear.
Methods: In this study, we used JBZ-DH5α (which overexpresses BcsZ on the E.
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