The analysis of sixteen Komagataea (Williopsis) pratensis from the Caucasian and Tien Shan soils by the PCR, blot hybridization, and isoenzyme electrophoresis techniques showed that fifteen of them do belong to the species K. pratensis. The isolates from the two geographic areas differed in some physiological characteristics and in the PCR product profiles obtained with the microsatellite primers (CAC)5 and (GACA)4.
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Fifty-three strains of saturn-spored yeasts were analyzed by means of restriction analysis of the amplified fragment of rDNA which comprised the 5.8S rRNA gene and the internal transcribed spacers ITS1 and ITS2. The use of endonucleases HaeIII and MspI enabled clear differentiation of yeast species Williopsis mucosa, W.
View Article and Find Full Text PDFDifferentiation of the yeasts Williopsis, Zygowilliopsis and Komagataea by karyotypic and PCR analyses.
Syst Appl Microbiol
March 2004
State Institute for Genetics, Selection of Industrial Microorganisms, Moscow, Russia.
We used polymerase chain reaction with universal and microsatellite primers, and molecular karyotyping to evaluate the extent of divergence between the genomes of the yeasts currently assigned to the heterogeneous genus Williopsis. Pulsed-field gel electrophoresis of chromosomal DNAs indicates that Zygowilliopsis californica, Komagataea pratensis, Williopsis mucosa, Williopsis salicorniae species and Williopsis sensu stricto complex have clearly different karyotypes. In contrast, the latter six species, Williopsis saturnus, W.
View Article and Find Full Text PDF[Molecular-genetic analysis of the yeast Komagataea (williopsis) pratensis populations from Caucasian and Tien Shan regions].
Mikrobiologiia
December 2001
State Research Institute of Genetics and Selection of Industrial Microorganisms, Pervyi Dorozhnyi proezd 1, Moscow, 113545 Russia.
The analysis of sixteen Komagataea (Williopsis) pratensis from the Caucasian and Tien Shan soils by the PCR, blot hybridization, and isoenzyme electrophoresis techniques showed that fifteen of them do belong to the species K. pratensis. The isolates from the two geographic areas differed in some physiological characteristics and in the PCR product profiles obtained with the microsatellite primers (CAC)5 and (GACA)4.
View Article and Find Full Text PDFThe phylogenetic relationships of the saturn-shaped ascospore-forming species of the genus Williopsis Zender and related genera based on the partial sequences of 18S and 26S ribosomal RNAs (Saccharomycetaceae): the proposal of Komagataea Gen. Nov.
Biosci Biotechnol Biochem
July 1994
Department of Agricultural Chemistry, Faculty of Agriculture, Shizuoka University, Japan.
The partial base sequences of 18S and 26S rRNAs of strains of Williopsis and Saturnospora species were analyzed. In the three regions partially sequenced, the higher base differences were observed in the strains examined of the three species, W. californica, W.
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