[Determination of the rate constants for the interaction of enzymes with substrates and inhibitors during a short incubation time].

Kinetics of enzymatic reactions was studied using equipment which permitted to decrease the time of incubation of an enzyme with substrate or inhibitor up to two sec; principles of action, design and experimental procedure are described. A method of pipetting under pressure was employed to accelerate an addition of suitable reagents to the enzyme solution and for rapid stirring of the reaction mixture. Time of incubation was automatically measured with a precision of plus or minus 0.01 sec using an electronic stop-watch. Start and stop of the watch were made by means of electric gauges with platinum electrodes, placed into the channels through which the substrate or inhibitor solutions were supplied to the reaction mixture.