AI Article Synopsis
- Exposure of LNCaP prostate cancer cells to Ukrain (NSC-631570) significantly inhibits cell growth and induces apoptosis, particularly after 24 hours of treatment with 3.5 microM, resulting in 73% of cells entering the G2/M phase.
- At higher concentrations (7 microM and 17.5 microM), there were fewer dramatic changes, but G2/M accumulation remained observable, with apoptotic cell rates increasing in a dose-dependent manner, reaching 20% at 17.5 microM.
- Analysis of cyclins, cyclin-dependent kinases (CDK), and apoptosis-related proteins revealed changes in CDK1 and CDK2 levels, along with an increase in the CDK inhibitor
Article Abstract
Exposure of LNCaP prostate cancer cells to Ukrain (NSC-631570), a novel semisynthetic drug from Chelidonium majus L., results in cell growth inhibition which is concomitant with apoptosis. After 24 h treatment with 3.5 microM of Ukrain as many as 73% cells were found in the G2/M phase. However, at higher drug concentrations (7 microM and 17.5 microM) the changes in cell phase distribution were less dramatic but cell accumulation in the G2/M phase was still evident. The rate of apoptotic cells rose steadily with increased drug concentration in a dose-dependent manner and reached 20% at a dosage of 17.5 microM. To investigate whether the cell cycle control mechanisms are affected in response to Ukrain, we analyzed the expression levels of some cyclins, cyclin-dependent kinases (CDK) and apoptosis-related proteins in drug treated cancer cells. Western blot experiments revealed alterations in levels of CDK1 and CDK2, after treatment. Up-regulation of the CDK inhibitor p27 was observed, which may lead to G2/M cell accumulation, but no substantial changes in expression of Bcl-2 and Bax proteins were found.
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