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Expanded HPV Genotyping by Single-Tube Nested-Multiplex PCR May Explain HPV-Related Disease Recurrence.
Microorganisms
November 2024
Laboratory of Genetics and Molecular Biology, Department of Biology, Federal University of Maranhão, São Luís 65085-580, MA, Brazil.
The role of the human papillomavirus (HPV) in the establishment of cervical cancer has driven studies to find more effective methods of viral detection so that early intervention strategies can be performed. However, the methods still have limitations, especially regarding detecting the different genotypes simultaneously. We have developed a high-throughput system using a single-tube nested-multiplex polymerase chain reaction (NMPCR) for the detection of 40 HPV genotypes using capillary electrophoresis.
View Article and Find Full Text PDFSeven Theorems of Joseph G. Gall.
Exp Cell Res
January 2025
School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, OX1 3PT, UK. Electronic address:
On June 30, 2020, Professor Joseph Grafton Gall announced his retirement at 92. On August 13, 2020, Joe's former trainees and colleagues held a retirement celebration online to celebrate Joe's "Remarkable Career with Astonishing Discoveries", covering Joe's nearly 70 years of education and research. As a representative of Joe's trainees in the 2000s, I gave a speech titled "Seven Theorems of Joe".
View Article and Find Full Text PDFIn the last issue of (JPE 32.4), we dedicated a special column "In Memoriam: Judith Lothian, PhD, RN, LCCE, FAAN, FACCE," the associate editor of JPE. In this issue of the journal, our dedication continues with the editor and a number of Judy's close friends and colleagues sharing some special reflection to remember Judy.
View Article and Find Full Text PDFColin Dawes In Memoriam: Editor Emeritus and Pioneer in Salivary Research.
J Dent Res
November 2024
College of Dentistry, University of Saskatchewan, Saskatoon, Canada
Alternative Splicing of the Last Intron Yields Transcripts Differentially Expressed in Human Tissues That Code In Vitro for a Protein Devoid of Triokinase and FMN Cyclase Activity.
Biomolecules
October 2024
Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Medicina, Universidad de Extremadura, 06006 Badajoz, Spain.
The 18-exon human gene codes for dual-activity triokinase and FMN cyclase (TKFC) in an ORF, spanning from exon 2 to exon 18. In addition to TKFC-coding transcripts (classified as type by their intron-17 splice), databases contain evidence for alternative transcripts, but none of them has been expressed, studied, and reported in the literature. A novel full-ORF transcript was cloned from brain cDNA and sequenced (accession no.
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