Peach GF305 is frequently used as rootstock in experiments to evaluate the resistance of different species of Prunus to Plum pox virus (PPV) because of its extreme susceptibility. However, transmission of PPV in Prunus species is sometimes problematic due to its low concentration or uneven distribution in these species. To determine the most effective way of transmitting the virus, different infection methods (by aphids, grafting, mechanical infection and injection) were tested using Dideron PPV isolates. The most effective method was the grafting of herbaceous material with inoculum derived from similar herbaceous material. Infection by aphids was more laborious and less effective than grafting, showing many disadvantages. Neither mechanical infection nor injection transmitted the virus.
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Int J Mol Sci
December 2024
Department of Plant Breeding, CEBAS-CSIC, Espinardo, P.O. Box 164, 30100 Murcia, Spain.
Sharka disease, caused by the plum pox virus (PPV), negatively impacts stone fruit production, resulting in economic losses. It has been demonstrated that grafting the almond ( (Miller) D.A.
View Article and Find Full Text PDFPlant Dis
July 2024
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Peach latent mosaic viroid (PLMVd) infects peach trees in China and induces a conspicuous albino phenotype (peach calico, PC) that is closely associated with variants containing a 12-to-14 nucleotide hairpin insertion capped by a U-rich loop. Initially, PC disease distribution was limited to parts of Italy, and it was first detected in the field in China in 2019. To explore the molecular and biological characteristics of PLMVd PC isolates in peach in China, we conducted a comprehensive analysis of disease phenotype development and investigated the data-associated pathogenicity and in vivo dynamics of the Chinese isolate PC-A2 using slash-inoculation into GF-305 peach seedlings.
View Article and Find Full Text PDFFront Plant Sci
October 2022
UMR Qualisud, Avignon Université, Avignon, France.
The transcriptomic and metabolomic responses of peach to infestation were studied in Rubira, an accession carrying the major resistance gene causing antixenosis, and GF305, a susceptible accession. Transcriptome and metabolome showed both a massive reconfiguration in Rubira 48 hours after infestation while GF305 displayed very limited changes. The Rubira immune system was massively stimulated, with simultaneous activation of genes encoding cell surface receptors involved in pattern-triggered immunity and cytoplasmic NLRs (nucleotide-binding domain, leucine-rich repeat containing proteins) involved in effector-triggered immunity.
View Article and Find Full Text PDFPhytopathology
February 2023
Université de Bordeaux, INRAE, UMR BFP, Villenave d'Ornon, France.
Members of the genus are responsible for economically destructive plant diseases worldwide. Over the past few years, three luteoviruses infecting trees have been characterized. However, the biological properties, prevalence, and genetic diversity of those viruses have not yet been studied.
View Article and Find Full Text PDFPhytopathology
September 2022
Department of Plant Breeding, CEBAS-CSIC, 30100 Murcia, Spain.
(PPV) causes sharka disease in trees. Peach () trees are severely affected by PPV, and no definitive source of genetic resistance has been identified. However, previous results showed that PPV-resistant 'Garrigues' almond () was able to transfer its resistance to 'GF305' peach through grafting, reducing symptoms and viral load in PPV-infected plants.
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